Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China

被引:0
作者
Wei Gao
Xiangxing Gao
Tiezhu Mi
Bin Han
Yiran Zhang
Xinzi Li
Xiaofei Yin
Chengjun Sun
Qian Li
Zhisong Cui
Xiao Luan
Zhigang Yu
Li Zheng
机构
[1] Ocean University of China,College of Marine Life Sciences
[2] Ministry of Natural Resources,Marine Ecology Research Center, First Institute of Oceanography
[3] National Deep Sea Center,College of Environmental Science and Engineering
[4] Ocean University of China,Key Laboratory of Marine Chemical Theory and Technology
[5] Ministry of Education,Laboratory for Marine Ecology and Environmental Science
[6] Pilot National Laboratory for Marine Science and Technology (Qingdao),undefined
来源
Acta Oceanologica Sinica | 2019年 / 38卷
关键词
oil spill; biodegradation; microbial consortium; dominant bacteria;
D O I
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中图分类号
学科分类号
摘要
A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oil-degrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems.
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页码:54 / 64
页数:10
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[41]  
Kostka J E(undefined)A survey of undefined undefined undefined-undefined
[42]  
Hazen T C(undefined) (DWH) oil-degrading bacteria from the Eastern oyster biome and its surrounding environment undefined undefined undefined-undefined
[43]  
Kleindienst S(undefined)Indigenous oil-degrading bacteria in crude oil-contaminated seawater of the Yellow sea, China undefined undefined undefined-undefined
[44]  
Paul J H(undefined)Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent undefined undefined undefined-undefined
[45]  
Joye S B(undefined)undefined undefined undefined undefined-undefined
[46]  
Kostka J E(undefined)undefined undefined undefined undefined-undefined
[47]  
Prakash O(undefined)undefined undefined undefined undefined-undefined
[48]  
Overholt W A(undefined)undefined undefined undefined undefined-undefined
[49]  
Lai Q(undefined)undefined undefined undefined undefined-undefined
[50]  
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