Two copies of blaNDM-1 gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia

被引:0
作者
Branko Jovčić
Zorica Lepšanović
Jelena Begović
Brankica Filipić
Milan Kojić
机构
[1] University of Belgrade,Institute of Molecular Genetics and Genetic Engineering
[2] University of Belgrade,Faculty of Biology
[3] Military Medical Academy,Faculty of Pharmacy
[4] University of Belgrade,undefined
来源
Antonie van Leeuwenhoek | 2014年 / 105卷
关键词
NDM-1; Metallo-β-lactamase; Molecular genetics;
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摘要
New Delhi metallo-β-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the blaNDM-1 gene. Restriction analysis and hybridization revealed that two copies of the blaNDM-1 gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the blaNDM-1 gene in the genome of P. aeruginosa MMA83.
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页码:613 / 618
页数:5
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