Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43

被引：0
作者：
Balakrishnan Divya
S. Robin
R. Rabindran
S. Senthil
M. Raveendran
A. John Joel
机构：
[1] Tamil Nadu Agricultural University,Department of Plant Genetic Resources, Centre for Plant Breeding and Genetics
[2] Tamil Nadu Agricultural University,Department of Rice, Centre for Plant Breeding and Genetics
[3] Tamil Nadu Agricultural University,Department of Plant Pathology, Centre for Plant Protection Studies
[4] Tamil Nadu Agricultural University,Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology and Biotechnology
[5] Tamil Nadu Agricultural University,Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology
[6] Directorate of Rice Research-ICAR,ICAR
来源：
Euphytica | 2014年 / 200卷
关键词：
Rice; Blast resistance; Gene pyramiding; MAS; Backcross breeding;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Marker assisted backcrossing breeding has become one of the essential tools in transferring novel genes to adapted varieties and was employed to pyramid three blast resistance genes Pi1, Pi2 and Pi33 to a popular susceptible rice variety ADT43. Gene pyramiding process was facilitated by marker aided selection for both foreground as well as background genotype. Previously reported linked molecular markers were deployed to survey resistant and susceptible genotypes. In the BC3F1 generation four lines viz, AC-B3-11-7, AC-B3-11-36, AC-B3-11-57, AC-B3-11-83 were identified to be pyramided with three genes and subjected to background analysis and a genome recovery up to 95 % was observed and advanced to further generations. Morphological, yield and grain quality traits were significantly similar to ADT43. The introgressed lines with three gene combinations were highly resistant to the blast pathogen compared to genotypes with single genes and the susceptible checks under blast nursery screening at two epiphytotic locations; Coimbatore and Gudalur. The selected three gene pyramided backcross lines in the desirable background were advanced to obtain an improved ADT 43 with resistance to blast disease.
引用
收藏
页码：61 / 77
页数：16
相关论文
共 343 条
  • [1] Alvarez A(2007)Genetic diversity analysis of Cuban traditional rice ( Genet Mol Biol 30 1109-1117
  • [2] Fuentes JL(2005) L.) varieties based on microsatellite markers Curr Sci 89 930-931
  • [3] Puldon V(1977)Rice blast fungus sequenced Mycologia 69 952-956
  • [4] Barman SR(2010)Magnaporthe, telimenella and hyponectria (Physosphorellaceae) Mol Breed 26 293-305
  • [5] Chattoo BB(2006)Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid Biotechnol Agron Soc Env 10 77-81
  • [6] Barr ME(2005)Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels J Biotechnol Pertaninan 10 51-54
  • [7] Basavaraj SH(1997)Application of molecular marker for the assessment of blast resistance genes, Theor Appl Genet 95 553-567
  • [8] Singh VK(2004) and Digu J Phytopathol 152 77-85
  • [9] Singh A(2003), incorporated into Indonesian rice cultivars Theor Appl Genet 107 1014-1020
  • [10] Singh A(2002)Development of a microsatellite framework map providing genome-wide coverage in rice ( Fitopatol Colomb 26 47-54
12345678910