Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium pahalae)

被引：50
作者：
Fang Y. [1 ]
Smith M.A.L. [1 ,2 ]
Pépin M.-F. [1 ]
机构：
[1] Dept. Nat. Rsrc. and Environ. Sci., University of Illinois, Urbana, IL 61801
[2] 1021 Plant Sciences Lab MC 634, Dept. NRES, Univ. of Illinois, Urbana, IL 61801
来源：
In Vitro Cellular & Developmental Biology - Plant | 1999年 / 35卷 / 1期
关键词：
Ibuprofen; Inhibitor; Signal transduction; Signaling compound;
D O I：
10.1007/s11627-999-0019-8
中图分类号：
学科分类号：
摘要：
Elicitation of anthocyanin-producing cells of ohelo (Vaccinium pahalae) by both biotic (purified β-glucan and chitosan) and abiotic [sodium ferric ethylenediamine di-(o-hydroxyphenylacetate) FeEDDHA, and CuSO4] elicitors resulted in significant enhancement of anthocyanin accumulation. Anthocyanin production increased up to 1.8 and 1.5-fold over the control in the presence of abiotic elicitors (90 μM FeEDDHA and 20 μM CuSO4, respectively), and increased 1.9 and 1.6-fold in the presence of biotic elicitors (10 mg L-1 β-glucan and 100 mg L-1 chitosan). Maximum anthocyanin production with the two most effective elicitors was achieved when cultures were treated on Day 3 (β-glucan) or Day 0 (FeEDDHA) after the initiation of fresh cell cultures. A concentration-dependent response was exhibited by cultures treated with exogenous methyl jasmonate (MJ). The addition of 0.5 μM MJ alone provoked a 2-3-fold increase in anthocyanin production over that of the control; however, no additive effect on anthocyanin production was observed in any treatments which combined MJ and β-glucan or FeEDDHA. Conditioning of the cells with a preculture in either MJ, β-glucan, or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures elicited by MJ or β-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather than inhibited, anthocyanin production, resulting in levels of accumulation beyond any of the tested elicitor combinations. Hypotheses for the observed influence of ibuprofen in this system are discussed.
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页码：106 / 113
页数：7
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