Bio-efficiency of Antifungal Lactic Acid Bacterial Isolates for Pomegranate Fruit Rot Management

被引：5

作者：

Gajbhiye M. ^{[1
]}

Kapadnis B. ^{[2
]}

机构：

[1] Department of Microbiology, Tuljaram Chaturchand College, Baramati

[2] Department of Microbiology, Savitribai Phule Pune University, Pune

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2018年 / 88卷 / 4期

关键词：

Antifungal; Biocontrol; Fruit rot; Lactic acid bacteria; Pomegranate;

D O I：

10.1007/s40011-017-0891-7

中图分类号：

学科分类号：

摘要：

Out of 360 lactic acid bacteria (LAB) from leaves, flowers, buds and fruits of pomegranate, 224 were antagonistic towards Aspergillus flavus. In all, 81.6% of antifungal LAB were cocci and 18.3% were rod shaped. Majority (39.7%) of them was obtained from flowers and 27 isolates showed strong antifungal activity. Antifungal isolates were identified by Gram positive card kit and 16S rRNA gene sequencing as Enterococcus faecium, E. durans, Lactococcus lactis spp. cremoris, L. lactis spp. lactis, Leuconostoc mesenteroides, Lactobacillus brevis and Pediococcus pentosaceus. All the isolates showed maximum antifungal activity at pH 6.5 and 30 °C on MRS agar. The antifungal activity of cell free supernatants from the entire isolates was same under static and shaking conditions except PFL9 that showed decreased production under shaking conditions. The isolates, PFL4, PFL9, PB6, PB119, PL14, PFR61 and PFR77 showed broad antifungal activity. PFL9 and PFR77 had the longest survival on fruits and there was no significant reduction (P > 0.05) in their population till 8th day post inoculation as determined under field conditions. Cell formulations of PFL9 and PFR77, and Dithane (fungicide preparation) delayed the development of rot caused by Alternaria alternata, Phomopsis varsoniana and Corynespora cassiicola with equivalent potential (P > 0.05). Thus, pomegranate is the source of diverse antifungal LAB which can be used as an effectual biocontrol agent in the management of fungal fruit rot of pomegranate. © 2017, The National Academy of Sciences, India.

引用

页码：1477 / 1488

页数：11

共 27 条

[1]

Somasekhara Y.M., Disease notes—New record of Ceratocystis fimbriata causing wilt of pomegranate in India, Plant Dis, 83, (1999)

[2]

Sheikh M.K., The pomegranate, (2006)

[3]

Tziros G.T., Lagopodi A.L., Tzavella-Klonari K., Alternaria alternata fruit rot of pomegranate (Punica granatum) in Greece, Plant Pathol, 57, (2008)

[4]

Tziros G.T., Tzavella-Klonari K., Pomegranate fruit rot caused by Coniella granati confirmed in Greece, Plant Pathol, 57, (2008)

[5]

Compant S., Duffy B., Nowak J., Clement C., Use of plant growth promoting bacteria for biocontrol of plant diseases: principles, mechanism of action, and future prospects: minireview, Appl Environ Microbiol, 71, pp. 4951-4959, (2005)

[6]

Sathe S.J., Nawani N.N., Dhakephalkar P.K., Kapadnis B.P., Antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables, J Appl Microbiol, 103, pp. 2622-2628, (2007)

[7]

Magnusson J., Strom K., Roos S., Sjogren J., Schnurer J., Broad and complex antifungal activity among environmental isolates of lactic acid bacteria, FEMS Microbiol Lett, 219, pp. 129-135, (2003)

[8]

Laitila A., Alakomi H.-L., Mattila-Sandholm T., Haikara A., Antifungal activities of two Lactobacillus plantarum strains against Fusarium molds in vitro and in malting of barley, J Appl Microbiol, 93, pp. 566-576, (2002)

[9]

Rouse S., Harnett D., Vaughan A., van Sinderen D., Lactic acid bacteria with potential to eliminate fungal spoilage in foods, J Appl Microbiol, 104, pp. 915-923, (2008)

[10]

Strom K., Sjogren J., Broberg A., Schnurer J., Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo (L-phe-L-pro) and cyclo (L-phe-trans-4-oh-L-pro) and 3-phenyllactic acid, Appl Environ Microbiol, 68, pp. 4322-4327, (2002)

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