A procedure for determining triglycerides of vegetable oils by microcolumn reversed-phase HPLC with UV detection at 210 nm was developed. Acetonitrile and diethyl ether mixtures (10 : 4–6.5, by volume) were proposed as eluants. The procedure was applied to the identification of oils (linseed, corn, olive, sunflower, and pumpkin oils), to the detection of adulterated oils (using olive and sea-buckthorn oils as examples), and to the determination of the degree of the unsaturation of oils.