Effects of electron donors on nitrate removal by nitrate and nitrite reductases
被引:7
作者:
Sung D.W.
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机构:
School of Chemical Engineering, Seoul National UniversitySchool of Chemical Engineering, Seoul National University
Sung D.W.
[1
]
Song S.H.
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机构:
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National UniversitySchool of Chemical Engineering, Seoul National University
Song S.H.
[2
]
Kim J.H.
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机构:
Biotechnology and Chemicals Industries Division, Ministry of Commerce, Industry and EnergySchool of Chemical Engineering, Seoul National University
Kim J.H.
[3
]
Yoo Y.J.
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机构:
School of Chemical Engineering, Seoul National UniversitySchool of Chemical Engineering, Seoul National University
Yoo Y.J.
[1
]
机构:
[1] School of Chemical Engineering, Seoul National University
[2] Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University
[3] Biotechnology and Chemicals Industries Division, Ministry of Commerce, Industry and Energy
Denitrification;
Electron donor;
Nitrate reductase;
Nitrite reductase;
Ochrobactrum antropi;
D O I:
10.1007/BF02935889
中图分类号:
学科分类号:
摘要:
Effects of artificial electron donors to deliver reducing power on enzymic denitrification were investigated using nitrate reductase and nitrite reductase obtained from Ochrobactrum antropi. The activity of nitrite reductase in the soluble portion was almost the same as that in the precipitated portion of the cell extract. Nitrate removal efficiency was higher with benzyl viologen than with methyl viologen or NADH as an artificial electron donor. The turn-over numbers of nitrate and nitrite reductase were 14.1 and 1.9 μmol of nitrogen reduced/min · mg cell extracts, respectively when benzyl viologen was used as an electron donor.
机构:
Univ Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, CanadaUniv Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, Canada
Badawi, AF
Hosny, G
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Univ Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, CanadaUniv Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, Canada
Hosny, G
El-Hadary, M
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机构:
Univ Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, CanadaUniv Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, Canada
El-Hadary, M
Mostafa, MH
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机构:
Univ Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, CanadaUniv Prince Edward Isl, Lab Pharmacogenet, Charlottetown, PE C1A 4P3, Canada