Effects of electron donors on nitrate removal by nitrate and nitrite reductases

被引：7
作者：
Sung D.W. [1 ]
Song S.H. [2 ]
Kim J.H. [3 ]
Yoo Y.J. [1 ]
机构：
[1] School of Chemical Engineering, Seoul National University
[2] Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University
[3] Biotechnology and Chemicals Industries Division, Ministry of Commerce, Industry and Energy
来源：
Biotechnology and Bioprocess Engineering | 2002年 / 7卷 / 2期
关键词：
Denitrification; Electron donor; Nitrate reductase; Nitrite reductase; Ochrobactrum antropi;
D O I：
10.1007/BF02935889
中图分类号：
学科分类号：
摘要：
Effects of artificial electron donors to deliver reducing power on enzymic denitrification were investigated using nitrate reductase and nitrite reductase obtained from Ochrobactrum antropi. The activity of nitrite reductase in the soluble portion was almost the same as that in the precipitated portion of the cell extract. Nitrate removal efficiency was higher with benzyl viologen than with methyl viologen or NADH as an artificial electron donor. The turn-over numbers of nitrate and nitrite reductase were 14.1 and 1.9 μmol of nitrogen reduced/min · mg cell extracts, respectively when benzyl viologen was used as an electron donor.
引用
收藏
页码：112 / 116
页数：4
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