Preproenkephalin-like Immunoreactive and Calcium-binding Proteins-like Immunoreactive Double-labelled Neurons in the Spinal Trigeminal Nucleus Caudalis of the Rat

被引：0
作者：
Ya-Yun Wang
Sheng-Xi Wu
Yun-Qing Li
机构：
[1] The Fourth Military Medical University,Department of Anatomy and K.K. Leung Brain Research Centre
来源：
The Histochemical Journal | 2002年 / 34卷
关键词：
Neuronal Cell; Cell Body; Neuronal Cell Body; Trigeminal Nucleus; Immunoreactive Neuron;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Immunofluorescence histochemical double-staining for preproenkephalin (PPE) and calbindin-D28k (CB), calretinin (CR) or parvalbumin (PV) were performed in the spinal trigeminal nucleus caudalis (Vc) of the rat. Neuronal cell bodies exhibiting PPE-like immunoreactivity were present in all laminae of the Vc, with a higher concentration in lamina II. Most of the CB-, CR- and PV-like immunoreactive neurons were located in lamina II, and some of them were also found in laminae I and III of the Vc. Some PPE-like immunoreactive neurons also showed CB-, CR-, or PV-like immunoreactivities. CB/PPE, CR/PPE and PV/PPE double-labelled neurons were mainly observed in lamina II. The percentages of CB/PPE double-labelled neurons in the total numbers of the CB- and PPE-like immunoreactive neurons were 3.5–1.5% and 3.3–15.7%, respectively. Of all CR- and PPE-like immunoreactive neurons, 4.7–13.5% and 3.7–14.2% showed both CR- and PPE-like immunoreactivities. The ratios of PV/PPE double-labelled neurons in all PV- and PPE-like immunoreactive neurons were 9.7–28.1% and 2.1–8.7%, respectively. The present results indicate that some enkephalinergic neurons in the Vc of the rat also contain calcium-binding proteins.
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页码：241 / 245
页数：4
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共 56 条
[1]  
Baimbridge KG(1992)Calcium-binding proteins in the nervous system Trends Neurosci 15 303-308
[2]  
Celio MR(1984)Endogenous pain control systems: Brainstem spinal pathways and endorphin circuitry Annu Rev Neurosci 7 309-338
[3]  
Rogers JH(1992)Parvalbumin and calbindin immunocytochemistry reveal functionally distinct cell groups and vibrissa-related patterns in the trigeminal brainstem complex of the adult rat J Comp Neurol 320 323-338
[4]  
Basbaum AI(1986)Parvalbumin in most gamma-aminobutyric acidcontaining neurons of the rat cerebral cortex Science 231 995-7
[5]  
Fields HL(1983)Spinal and trigeminal mechanisms of nociception Annu Rev Neurosci 6 381-418
[6]  
Bennett-Clarke CA(1987)Localization of preproenkephalin mRNA in the rat brain and spinal cord by J Comp Neurol 258 159-184
[7]  
Chiaia NL(1994) hybridization Neuroscience 61 123-132
[8]  
Jacquin MF(1997)Subpopulations of GABAergic neurons in laminae I-III of rat spinal dorsal horn defined by coexistence with classical transmitters, peptides, nitric oxide synthase or parvalbumin J Comp Neurol 386 229-244
[9]  
Rhoades RW(1999)Preprodynorphin-, preproenkephalin-, and preprotachykinin-expressing neurons in the rat neostriatum: An analysis by immunocytochemistry and retrograde tracing Neurosci Res 35 225-240
[10]  
Celio MR(1999)Calcium-binding protein-like immunoreactive projection neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat J Comp Neurol 411 399-412
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