The Role of Long Non-Coding RNA CCAT1 and SNHG14 in Activation of Some Protein-Coding Genes Associated with the Development of Ovarian Cancer

被引：0
作者：
O. I. Brovkina
I. V. Pronina
A. M. Burdennyy
L. A. Uroshlev
E. A. Filippova
M. V. Fridman
T. P. Kazubskaya
K. I. Zhordania
V. I. Loginov
N. E. Kushlinskii
E. A. Braga
机构：
[1] Research Institute of General Pathology and Pathophysiology,Federal Research Clinical Center of Specialized Types of Medical Care and Medical Technologies
[2] Federal Medical-Biological Agency of Russia,N. N. Blokhin National Medical Research Center of Oncology
[3] N. I. Vavilov Institute of General Genetics,undefined
[4] Russian Academy of Sciences,undefined
[5] Ministry of Health of the Russian Federation,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2022年 / 172卷
关键词：
ovarian cancer; long non-coding RNA; microRNA; epithelial-mesenchymal transition; biomarkers;
D O I：
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中图分类号：
学科分类号：
摘要：
Late diagnosis of ovarian cancer is one of the most important problems in its treatment. Long non-coding RNA (lncRNA) are a poorly studied, but promising type of diagnostic biomarkers. We studied the lncRNA interactome to identify biomarkers with potential significance for molecular diagnostics of ovarian cancer. By screening the TCGA database, we identified differentially expressed lncRNA CCAT1 and SNHG14. Based on the indices of complementarity of CCAT1 and SNHG14 to the mRNA sequences, we selected 5 protein-coding genes MAPK1, c-MET, TGFB2, SNAIL1, and WNT4 associated with the epithelial-mesenchymal transition. Real-time PCR on 54 ovarian cancer samples confirmed the high expression levels of CCAT1 and SNHG14 (logFC>1.5, p<0.05). A positive correlation between the expression levels of two lncRNA and mRNA of 5 genes in 6 pairs was established. The activating effect of CCAT1 and SNHG14 on the expression of these genes can be mediated by miR-203 and miR-124.
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页码：760 / 764
页数：4
相关论文
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  • [1] Brovkina OI(2021)Identification of novel differentially expressing long non-coding RNAs with oncogenic potential Mol. Biol. 55 548-554
  • [2] Pronina IV(2021)Changes in the methylation level of microRNA genes as a factor of breast cancer development and progression Patol. Fiziol. Eksp. Ter. 65 4-11
  • [3] Uroshlev LA(2017)Long non-coding RNA CCAT1 promotes metastasis and poor prognosis in epithelial ovarian cancer Exp. Cell Res. 359 185-194
  • [4] Loginov VI(2015)Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge J. Exp. Clin. Cancer Res. 34 18-36
  • [5] Braga EA(2019)The functions and oncogenic roles of CCAT1 in human cancer Biomed. Pharmacother. 115 28-112
  • [6] Fridman MV(2018)Novel miRNA genes deregulated by aberrant methylation in ovarian carcinoma are involved in metastasis Gene. 662 102-1696
  • [7] Kazubskaya TP(2020)Epithelial-mesenchymal transition in cancer: a historical overview Transl. Oncol. 13 1685-269
  • [8] Utkin DO(2019)Non-redundant functions of EMT transcription factors Nat. Cell Biol. 21 259-27
  • [9] Kushlinskii NE(2020)Long noncoding RNA SNHG14 promotes the aggressiveness of retinoblastoma by sponging microRNA-124 and thereby upregulating STAT3 Int. J. Mol. Med. 45 17-531
  • [10] Filippova EA(2021)EMTome: a resource for pan-cancer analysis of epithelial-mesenchymal transition genes and signatures Br. J. Cancer. 124 513-4876
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