Characterization of a Novel Pollen-Specific Promoter from Wheat (Triticum Aestivum L.)

被引：0
作者：
Ling Chen
Yingjie Miao
Cheng Wang
Peipei Su
Tianheng Li
Rong Wang
Xinglong Hao
Guangxiao Yang
Guangyuan He
Chunbao Gao
机构：
[1] Institute of Food Crops,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement
[2] Hubei Academy of Agricultural Sciences,China
[3] Huazhong University of Science and Technology (HUST),UK HUST
来源：
Plant Molecular Biology Reporter | 2012年 / 30卷
关键词：
Wheat; Pollen-specific promoter; gene; Tobacco; Genetic transformation;
D O I：
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中图分类号：
学科分类号：
摘要：
PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.
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页码：1426 / 1432
页数：6
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