Rebamipide-induced downregulation of phospholipase D inhibits inflammation and proliferation in gastric cancer cells

被引：0
作者：
Dong Woo Kang
Gyesik Min
Do Yoon Park
Ki Whan Hong
Do Sik Min
机构：
[1] College of Natural Science,Department of Molecular Biology
[2] Pusan National University,Department of Pharmaceutical Engineering
[3] Busan 609-735,Department of Pathology
[4] Korea.,undefined
[5] Jinju National University,undefined
[6] Jinju 660-758,undefined
[7] Korea.,undefined
[8] College of Medicine,undefined
[9] Pusan National University,undefined
[10] Busan 609-739,undefined
[11] Korea.,undefined
[12] Medical Research Center for Ischemic Tissue Regeneration,undefined
[13] Pusan National University,undefined
[14] Yangsan 626-770,undefined
[15] Korea.,undefined
来源：
Experimental & Molecular Medicine | 2010年 / 42卷
关键词：
cell proliferation; gene expression regulation, neoplastic; inflammation; phospholipase D; rebamipide; stomach neoplasms;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Rebamipide a gastroprotective drug, is clinically used for the treatment of gastric ulcers and gastritis, but its actions on gastric cancer are not clearly understood. Phospholipase D (PLD) is overexpressed in various types of cancer tissues and has been implicated as a critical factor in inflammation and carcinogenesis. However, whether rebamipide is involved in the regulation of PLD in gastric cancer cells is not known. In this study, we showed that rebamipide significantly suppressed the expression of both PLD1 and PLD2 at a transcriptional level in AGS and MKN-1 gastric cancer cells. Downregulation of PLD expression by rebamipide inhibited its enzymatic activity. In addition, rebamipide inhibited the transactivation of nuclear factor kappa B (NFκB), which increased PLD1 expression. Rebamipide or PLD knockdown significantly suppressed the expression of genes involved in inflammation and proliferation and inhibited the proliferation of gastric cancer cells. In conclusion, rebamipide-induced downregulation of PLD may contribute to the inhibition of inflammation and proliferation in gastric cancer.
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页码：555 / 564
页数：9
相关论文
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