Ouabain switches Na+ transport to Na+/Na+ equivalent exchange in normal and apoptotic lymphoid cells

被引：0
作者：
Rubashkin A.A. [1 ]
机构：
[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg
来源：
Biophysics | 2013年 / 58卷 / 5期
关键词：
apoptosis; Na[!sup]+[!/sup] fluxes; Na[!sup]+[!/sup]/K[!sup]+[!/sup]-ATPase; Na[!sup]+[!/sup]/Na[!sup]+[!/sup] exchange; ouabain;
D O I：
10.1134/S000635091305014X
中图分类号：
学科分类号：
摘要：
A theory of change of the ionic fluxes in the lymphoid cells in their transition from normal to apoptosis we have developed previously is applied to the analysis of Na+/Na+ exchange fluxes in human lymphoid cells U937 exposed to ouabain. We solve a system of equations describing changes in the intracellular concentrations of Na+, K+ and Cl-, membrane potential and cell volume. It is shown that the Na+ influx (I Na/Na) and output flux through the Na+/Na+ tract increased 4 times in 8 h after disconnecting Na+/K+-ATPase for normal cell U937. These fluxes increased 2.6 times for apoptotic cells. The value of I Na/Na after 8 h off pump by ouabain is 97% of the total Na+ input for both cell types. It is concluded that ouabain not only inhibits the Na+/K+-ATPase, but also increases Na+ exchange fluxes through the Na+/Na+ tract, thereby switching sodium transport across the membrane of lymphoid cells to Na+/Na+ equivalent exchange. © 2013 Pleiades Publishing, Inc.
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页码：660 / 663
页数：3
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