Development and characterisation of microsatellite loci in Farfugium japonicum (Asteraceae)

被引：0
作者：
Naofumi Nomura
Kento Fujiwara
Tokushiro Takaso
Motomi Ito
Koichi Uehara
Hiroaki Setoguchi
机构：
[1] Research Institute for Humanity and Nature,Graduate School of Arts and Sciences
[2] Tokyo University,Graduate School of Horticulture
[3] Chiba University,Graduate School of Human and Environmental Studies
[4] Matsudo,undefined
[5] Kyoto University,undefined
来源：
Conservation Genetics | 2009年 / 10卷
关键词：
Conservation; Asteraceae; Heterozygosity; Microsatellite;
D O I：
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中图分类号：
学科分类号：
摘要：
Eight microsatellite loci for the perennial herb Farfugium japonicum, including the rheophytic variety luchuense endemic to riparian areas of the Ryukyu Islands, Japan, were isolated and characterised. The number of alleles ranged from 5 to 14. The expected (HE) and observed (HO) heterozygosities were 0.344–0.885 and 0.121–0.754, respectively, from 69 individuals in one population. Six loci exhibited significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). The primers amplifying microsatellite sequences in F. japonicum may provide a population genetics tool useful in the establishment of a conservation strategy.
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页码：1093 / 1095
页数：2
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