A human homolog of the yeast gene encoding tRNA 2′-phosphotransferase: cloning, characterization and complementation analysis

被引：0
作者：
Q.-D. Hu
H. Lu
K. Huo
K. Ying
J. Li
Y. Xie
Y. Mao
Y.-Y. Li
机构：
[1] Institute of Genetics,State Key Laboratory of Genetic Engineering
[2] School of Life Sciences,undefined
[3] Fudan University,undefined
来源：
Cellular and Molecular Life Sciences CMLS | 2003年 / 60卷
关键词：
Human ; tRNA splicing; DUF60 domain; Northern blot; functional complementation; plasmid shuffle;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The Saccharomyces cerevisiae TPT1 gene plays a role in removing the 2′-phosphate from ligated tRNA during the maturation of pre-tRNA. Here we reported the cloning and characterization of the human TRPT1 gene as a homolog of yeast TPT1. The TRPT1 gene is located at human chromosome 11q13 and encodes a polypeptide of 253 amino acids. BLAST searches with its amino acid sequence revealed the ubiquitous occurrence of TRPT1 homologs and their functional relationships with the presence of the DUF60/KptA domain. Northern analysis demonstrated that the gene is primarily expressed in heart and skeletal muscle, with lower or undetectable levels in other tissues studied. A plasmid-shuffling experiment showed that the human TRPT1 gene could complement the tpt1 mutation in S. cerevisiae
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页码：1725 / 1732
页数：7
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