Species identification of upstream fatminnow Rhynchocypris oxycephalus and downstream fatminnow Rhynchocypris lagowskii, based on PCR-RFLP of mitochondrial DNA

被引:0
作者
Noriyuki Takai
Yuta Abiko
Harumi Tsukamoto
Ai Miura
Kohei Yuasa
Shiro Itoi
Shizuko Nakai
Haruo Sugita
Kiyoshi Yoshihara
机构
[1] College of Bioresource Sciences,Department of Marine Science and Resources
[2] Nihon University,undefined
来源
Ichthyological Research | 2012年 / 59卷
关键词
Fatminnow; Species identification; PCR–RFLP; Izu Peninsula;
D O I
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中图分类号
学科分类号
摘要
The morphological similarity between upstream fatminnow Rhynchocypris oxycephalus and downstream fatminnow Rhynchocypris lagowskii makes it difficult to discriminate accurately between these species in rivers where they coexist. For easy and precise identification of these two species, we developed a genetic discrimination method based on PCR-RFLP analysis for specimens from the Inohzawa River watershed in the Izu Peninsula, central Honshu, Japan. This genetic method was applied to the species identification of the fatminnows from two other watersheds, the Kano and Kawazu Rivers, flowing across the peninsula from north to south. We present the genetic evidence for the restricted distribution of R. oxycephalus and the ubiquitous distribution of R. lagowskii in the peninsula.
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页码:156 / 163
页数:7
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