Production and Characterization of Cellulase-Free Xylanase from Trichoderma inhamatum

被引：0
|
作者：
Leonor Alves de Oliveira da Silva
Eleonora Cano Carmona
机构：
[1] São Paulo State University,Biochemistry and Microbiology Department, Bioscience Institute
[2] UNESP,undefined
来源：
Applied Biochemistry and Biotechnology | 2008年 / 150卷
关键词：
Xylanase; Optimization; Enzyme characterization; Enzyme regulation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The production of extracellular cellulase-free xylanase from Trichoderma inhamatum was evaluated in liquid Vogel medium with different carbon sources as natural substrates and agricultural or agro-industrial wastes. Optimal production of 244.02 U/mL was obtained with xylan as carbon source, pH 6.0 at 25°C, 120 rpm, and 60-h time culture. Optimal conditions for enzyme activity were 50°C and pH 5.5. Thermal stability of T. inhamatum xylanolytic complex expressed as T1/2 was 2.2 h at 40°C and 2 min at 50°C. The pH stability was high from 4.0 to 11.0. These results indicate possible employment of such enzymatic complex in some industrial processes which require activity in acid pH, wide-ranging pH stability, and cellulase activity absence.
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页码：117 / 125
页数：8
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