Co-encapsulation of pancreatic islets and pentoxifylline in alginate-based microcapsules with enhanced immunosuppressive effects

被引：29

作者：

Azadi S.A. ^{[1
]}

Vasheghani-Farahani E. ^{[1
]}

Hashemi-Najafbabadi S. ^{[1
]}

Godini A. ^{[2
]}

机构：

[1] Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran

[2] Department of Physiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah

来源：

Progress in Biomaterials | 2016年 / 5卷 / 2期

关键词：

Alginate; Dextran–spermine; Encapsulation; Pancreatic islets; Pentoxifylline;

D O I：

10.1007/s40204-016-0049-3

中图分类号：

学科分类号：

摘要：

Alginate-based scaffolds have received considerable attention for biomedical application because of their biocompatibility and ease of preparation. The application of alginate hydrogels for encapsulation of pancreatic islets is known as a potential treatment for type I diabetes. In this study, dextran–spermine coated microcapsules of alginate containing pancreatic islets were prepared, and then co-cultured with lymphocytes for 7 days. In addition, to prevent fibrosis and evaluating the effect of anti-inflammatory drugs, pentoxifylline was loaded in the inner layer of microcapsules. Intact and encapsulated islets in an external solution of pentoxifylline were taken as two separate controls in this study. Infrared and scanning electron microscope analyses showed polyelectrolyte complex formation between alginate and dextran–spermine. In vitro tests showed that interleukin-2 secretion from lymphocytes co-cultured with encapsulated islets containing pentoxifylline in the inner layer of microcapsules was 63.6 % lower than the corresponding value for encapsulated islets without the anti-inflammatory drug. © 2016, The Author(s).

引用

页码：101 / 109

页数：8

共 30 条

[1]

Aghajani-Lazarjani H., Vasheghani-Farahani E., Shojaosadati S.A., Hashemi-Najafabadi S., Zahediasl S., Tiraihi T., Atyabi F., The effect of two different polyethylene glycol (PEG) derivatives on the immunological response of PEG grafted pancreatic islets, J Artif Organs, 13, pp. 218-224, (2010)

[2]

Baruch L., Benny O., Gilert A., Ukobnik M., Itzhak O.B., Machluf M., Alginate–PLL cell encapsulation system co-entrapping PLGA-microspheres for the continuous release of anti-inflammatory drugs, Biomed Microdevices, 11, pp. 1103-1113, (2009)

[3]

Bensadoun J.-C., Pereira de Almeida L., Fine E.G., Tseng J.L., Deglon N., Aebischer P., Comparative study of GDNF delivery systems for the CNS: polymer rods, encapsulated cells, and lentiviral vectors, J Control Release, 87, pp. 107-115, (2003)

[4]

Berman B., Wietzerbin J., Sanceau J., Merlin G., Duncan M.R., Pentoxifylline inhibits certain constitutive and tumor necrosis factor-[agr]-induced activities of human normal dermal fibroblasts, J Investig Dermatol, 98, pp. 706-712, (1992)

[5]

Calafiore R., Basta G., Clinical application of microencapsulated islets: actual prospectives on progress and challenges, Adv Drug Deliv Rev, 67-68, pp. 84-92, (2014)

[6]

Chen G., Peng Y., Lou P., Liu J., Bioartificial thyroid. The in vitro culture of microencapsulated rabbit thyroid tissue, ASAIO Trans, 37, pp. M439-M440, (1990)

[7]

Christova-Bagdassarian V., Angelov T., Atanassova M., UV-spectrometric and high performance liquid chromatographic determination of pentoxifylline in workplace air, J Univ Chem Technol Metall, 42, pp. 223-227, (2007)

[8]

Chung T.W., Yang J., Akaike T., Cho K.Y., Nah J.W., Kim S.I., Cho C.S., Preparation of alginate/galactosylated chitosan scaffold for hepatocyte attachment, Biomaterials, 23, pp. 2827-2834, (2002)

[9]

Dang T.T., Et al., Enhanced function of immuno-isolated islets in diabetes therapy by co-encapsulation with an anti-inflammatory drug, Biomaterials, 34, pp. 5792-5801, (2013)

[10]

Darrabie M.D., Kendall W.F., Opara E.C., Characteristics of poly-l-ornithine-coated alginate microcapsules, Biomaterials, 26, pp. 6846-6852, (2005)

← 1 2 3 →