Molecular characterization of microalgae used in aquaculture with biotechnology potential

被引:0
作者
Mercedes Alonso
Fátima C. Lago
Juan M. Vieites
Montserrat Espiñeira
机构
[1] ANFACO-CECOPESCA,Area of Molecular Biology and Biotechnology
来源
Aquaculture International | 2012年 / 20卷
关键词
Molecular characterization; Microalgae; Aquaculture; PCR; 18S rRNA; 16S rRNA;
D O I
暂无
中图分类号
学科分类号
摘要
Microalgae are the main component of first tropic level in aquatic food chain; it is for this reason that they are used as food in aquaculture. Also due to its biotechnological potential properties, they are used in the production of diverse components, dyes, antioxidants, enzymes, emulsifiers, etc. The extended ways of microalgae applications require physiologically and genetically stable cultures as well as correctly identified organisms to guarantee reproducibility and reliability. But the variety of species and the morphological similarity between some of them make difficult the identification of some microalgae. The use of molecular markers has supplied a very useful tool for identification of microalgae in fast mode, such as in classification. The present study has worked on the molecular characterization of main species of microalgae used in aquaculture in base of the molecular markers 18S rRNA and 16S rRNA. Microalgae DNA has been amplified and sequenced, and the resultant sequences were analyzed and reflected in phylogenetic trees. The phylogenetic analyses obtained reflect as both molecular markers allow to differentiate the main genus used in aquaculture.
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页码:847 / 857
页数:10
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