Nitric oxide synthase inhibitor influences prostaglandin and interleukin-1 production in experimental arthritic joints

被引：0
作者：
De Mello S.B.V. [1 ]
Novaes G.S. [1 ]
Laurindo I.M.M. [1 ]
Muscará M.N. [2 ]
De Barros Maciel F.M. [1 ]
Cossermelli W. [1 ]
机构：
[1] Rheumatology Division, School of Medicine, University of São Paulo, São Paulo, 0124-6903 SP, Av. Dr. Arnaldo
[2] Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, 13081-970, Campinas SP
来源：
Inflammation Research | 1997年 / 46卷 / 2期
基金：
巴西圣保罗研究基金会;
关键词：
Eicosanoids; Experimental arthritis; Interleukin-1; Nitric oxide;
D O I：
10.1007/s000110050086
中图分类号：
学科分类号：
摘要：
Objective: To assess involvement of nitric oxide (NO) in the increase in eicosanoid and interleukin-1 (IL-1) levels in the synovial fluid during antigen-induced arthritis (AIA) in rabbits treated with a competitive inhibitor of NO synthesis. Subjects: Thirteen New Zealand White rabbits were sensitized with 5 mg of methylated bovine serum albumin (mBSA). Arthritis was induced in the knee joint by injecting 0.5 ml of a sterile solution of mBSA (2mg/ml) into the intra-articular cavity. Treatment: Prior to the induction of arthritis, the animals received N-Omega-Nitro-L-Arginine Methyl Ester (LNAME) or N-Omega-Nitro-D-Arginine Methyl Ester (DNAME) for 2 weeks, both at a dose of 20mg/kg/day mixed with drinking water. Methods: Leukocyte efflux (total and differential white cell count), vascular permeability (Evans's blue method), synovial PMN cell infiltrate, and total nitrite (NO2.)/nitrate (NO3.) (HPLC), PGE2, TxB2, LTB4 (radioimmunoassay), and IL-1β (ELISA) levels were quantified in the synovial fluid. Results: LNAME but not DNAME significantly suppressed leukocyte efflux and protein leakage into the articular cavity as well as synovial PMN cell infiltrate. Total N02./NO3., PGE2 and IL-1β levels were significantly reduced in the synovial fluid of LNAME treated animals. TxB2 and LTB4 were not affected by LNAME treatment. Conclusion: These data clearly show NO involvement in the IL-1-induced PGE2 production in the synovial fluid of antigen-induced arthritis in rabbits.
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页码：72 / 77
页数：5
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