Label-free detection of exosomes using a surface plasmon resonance biosensor

被引：0
作者：
Abu Ali Ibn Sina
Ramanathan Vaidyanathan
Alain Wuethrich
Laura G. Carrascosa
Matt Trau
机构：
[1] The University of Queensland,Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology
[2] National University of Singapore,Biomedical Institute for Global Health Research & Technology
[3] The University of Queensland,School of Chemistry and Molecular Biosciences
来源：
Analytical and Bioanalytical Chemistry | 2019年 / 411卷
关键词：
Exosome; Surface plasmon resonance; Biosensor; Cancer biomarker; Cancer diagnosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label-free detection of clinically relevant exosomes using a surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell–derived exosomes particularly from complex biological samples (e.g. exosome spiked in serum). This approach exhibits high sensitivity by detecting as low as 8280 exosomes/μL which may potentially be suitable for clinical analysis. We believe that this label-free and real-time method along with the high specificity and sensitivity may potentially be useful for clinical settings.
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页码：1311 / 1318
页数：7
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