A filter paper coated with phenylboronic acid-modified mesoporous silica for enrichment of intracellular nucleosides prior to their quantitation by HPLC

被引:0
作者
Ting Cheng
Yuan Zhang
Xiaoyan Liu
Xiaoyu Zhang
Haixia Zhang
机构
[1] Lanzhou University,State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, and College of Chemistry and Chemical Engineering
[2] Lanzhou University,Institute of Physiology, School of Basic Medical Sciences
来源
Microchimica Acta | 2017年 / 184卷
关键词
HPLC; Extraction; Capacity; SEM; -diol molecules; Boronate affinity materials; Gold nanoparticles; HepG2 cells;
D O I
暂无
中图分类号
学科分类号
摘要
The authors describe a filter paper modified with mesoporous silica that carries phenylboronic acid on its surface. The material possesses the advantages of the flexibility of filter paper and the large surface of mesoporous silica. This makes it an excellent sorbent for the enrichment of trace quantities of nucleosides. The limits of detection (in HPLC analysis with UV detection) are 2.0–11.1 μg L−1 and the limits of quantification are 6.8–37.1 μg L−1 with good precision (RSD < 6.4%). Compared to plain filter paper and nonporous silica-coated filter paper, the material exhibits enhanced efficiency and a 4.5-fold improved capacity. The potential applications are demonstrated by preconcentrating nucleosides from HepG2 cells which are a representative complex sample. The method was also used to study the effect of 30-nm gold nanoparticle on cellular metabolism.
引用
收藏
页码:4007 / 4013
页数:6
相关论文
共 180 条
[91]  
Bonnin C(undefined)undefined undefined undefined undefined-undefined
[92]  
Vignoles C(undefined)undefined undefined undefined undefined-undefined
[93]  
Gao L(undefined)undefined undefined undefined undefined-undefined
[94]  
Wang CZ(undefined)undefined undefined undefined undefined-undefined
[95]  
Wei YM(undefined)undefined undefined undefined undefined-undefined
[96]  
He XM(undefined)undefined undefined undefined undefined-undefined
[97]  
Zhu GT(undefined)undefined undefined undefined undefined-undefined
[98]  
Zhu YY(undefined)undefined undefined undefined undefined-undefined
[99]  
Chen X(undefined)undefined undefined undefined undefined-undefined
[100]  
Zhang Z(undefined)undefined undefined undefined undefined-undefined