A filter paper coated with phenylboronic acid-modified mesoporous silica for enrichment of intracellular nucleosides prior to their quantitation by HPLC

被引：0
作者：
Ting Cheng
Yuan Zhang
Xiaoyan Liu
Xiaoyu Zhang
Haixia Zhang
机构：
[1] Lanzhou University,State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, and College of Chemistry and Chemical Engineering
[2] Lanzhou University,Institute of Physiology, School of Basic Medical Sciences
来源：
Microchimica Acta | 2017年 / 184卷
关键词：
HPLC; Extraction; Capacity; SEM; -diol molecules; Boronate affinity materials; Gold nanoparticles; HepG2 cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The authors describe a filter paper modified with mesoporous silica that carries phenylboronic acid on its surface. The material possesses the advantages of the flexibility of filter paper and the large surface of mesoporous silica. This makes it an excellent sorbent for the enrichment of trace quantities of nucleosides. The limits of detection (in HPLC analysis with UV detection) are 2.0–11.1 μg L−1 and the limits of quantification are 6.8–37.1 μg L−1 with good precision (RSD < 6.4%). Compared to plain filter paper and nonporous silica-coated filter paper, the material exhibits enhanced efficiency and a 4.5-fold improved capacity. The potential applications are demonstrated by preconcentrating nucleosides from HepG2 cells which are a representative complex sample. The method was also used to study the effect of 30-nm gold nanoparticle on cellular metabolism.
引用
收藏
页码：4007 / 4013
页数：6
相关论文
共 180 条
  • [1] Liu Y(2011)A unique boronic acid functionalized monolithic capillary for specific capture, separation and immobilization of Chem Commun 47 5067-5069
  • [2] Ren L(2012)-diol biomolecules Chem Commun 48 4115-4117
  • [3] Liu Z(2015)A benzoboroxole-functionalized monolithic column for the selective enrichment and separation of cis-diol containing biomolecules Microchim Acta 182 185-192
  • [4] Li H(2017)A graphene oxide functionalized with 3-aminophenylboronic acid for the selective enrichment of nucleosides, and their separation by capillary electrophoresis Microchim Acta 184 271-278
  • [5] Wang H(2009)Surfactant assisted enrichment of nucleosides by using a sorbent consisting of magnetic polysulfone capsules and mesoporous silica nanoparticles modified with phenylboronic acid Analyst 134 2158-2164
  • [6] Liu Y(2012)Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins Microchim Acta 179 227-234
  • [7] Liu Z(2015)Electrochemical immunoassay for subgroup J of avian leucosis viruses using a glassy carbon electrode modified with a film of poly(3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody Microchim Acta 182 2615-2622
  • [8] Wang H(2017)A fluorescent turn-on detection scheme for α-fetoprotein using quantum dots placed in a boronate-modified molecularly imprinted polymer with high affinity for glycoproteins Microchim Acta 184 1463-1470
  • [9] Feng W(2017)A boronic acid based glucose assay based on the suppression of the inner filter effect of gold nanoparticles on the orange fluorescence of graphene oxide quantum dots Sensors Actuators B Chem 246 819-825
  • [10] Jia Q(2009)Glucose detection via glucose-induced disaggregation of ammonium-modified tetraphenylethylene from polyanion Angew Chem Int Ed 48 6704-6707
12345678910