Effect of differential concentration of micronutrient copper and zinc on in vitro morphogenesis of Foeniculum vulgare Mill.

被引:0
作者
Pratibha Dwivedi
Dhruti Amin
Abhishek Sharma
机构
[1] Biyani Girls College,Department of Science
[2] Uka Tarsadia University,C. G. Bhakta Institute of Biotechnology
来源
Plant Physiology Reports | 2020年 / 25卷
关键词
Mill.; Micropropagation; Micronutrients; Copper; Zinc;
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摘要
The present study was carried out to investigate the morphogenic roles played by copper and zinc during micropropagation of Foeniculum vulgare Mill., an aromatic medicinal herb and the major spices of traditional food. When grown on MS medium the cotyledonary node gave the maximum multiple shoot proliferation over MS medium supplemented with 1.0 mg L−1 BAP concentration. Incorporation of 0 μM CuSO4 or 29.91 µM ZnSO4 to Murashige and Skoog (MS) medium with optimized concentration of BAP i.e. 1.0 mg L−1 induced a maximum number of shoot buds per nodal explant (4.3 ± 0.8 and 4.1 ± 1.1, respectively). However, higher concentration of both the micronutrients effectively affected the morphogenic potential of F. vulgare. The optimum concentrations of Cu and Zn in culture media are highly important for obtaining rapid and enhanced in vitro multiplication of F. vulgare.
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页码:178 / 184
页数:6
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  • [1] Anzidei M(2000)Organogenesis and somatic embryogenesis in Plant Cell, Tissue and Organ Culture 61 69-79
  • [2] Bennici A(1996): Histological observations of developing embryogenic callus Plant Cell, Tissue and Organ Culture 45 263-268
  • [3] Schiff S(1939) culture of Plant Physiology 14 371-375
  • [4] Tani C(1995): Callus characteristics in relation to morphogenesis Water, Air, and Soil pollution 83 187-194
  • [5] Mori B(2014)The essentiality of certain elements in minute quantity for plants with special reference to copper BioMed Research International 2014 1-32
  • [6] Anzidei M(1987)Distribution of different fraction of cadmium, zinc, leads, and copper in unpolluted and polluted soil Plant Cell, Tissue and Organ Culture 10 101-113
  • [7] Vivona L(1995) Mill: A review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology Plant Cell, Tissue and Organ Culture 43 267-269
  • [8] Schiff S(2002)Induction of embryogenic ( Crop Science 42 934-938
  • [9] Bennici A(1991) L.) calli, I. quantification of cultivar and culture medium effects New Phytologist 118 565-570
  • [10] Arnon DI(1985)Improved plant regeneration from barley callus cultures by increased copper levels Plant Physiology 78 4-7