Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

被引：104

作者：

Rackova L. ^{[1
]}

Oblozinsky M. ^{[2
]}

Kostalova D. ^{[3
]}

Kettmann V. ^{[4
]}

Bezakova L. ^{[3
]}

机构：

[1] Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-841 04, Bratislava

[2] Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, SK-83232, Bratislava

[3] Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, SK-83232, Bratislava

[4] Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, SK-83232, Bratislava

来源：

Journal of Inflammation | / 4卷 / 1期

关键词：

Alkaloid; Berberine; Total Antioxidant Capacity; Antiradical Activity; Lipoxygenase Inhibition;

D O I：

10.1186/1476-9255-4-15

中图分类号：

学科分类号：

摘要：

Roots and stem-bark of Mahonia aquifolium (Oregon grape) (Berberidaceae) are effectively used in the treatment of skin inflammatory conditions. In the present study, the effect of Mahonia aquifolium crude extract and its two representative alkaloid fractions containing protoberberine and bisbenzylisoquinoline (BBIQ) alkaloids on activity of 12-lipoxygenase (12-LOX), was studied. The reactivity with 1,1-diphenyl-2-picryl-hydrazyl (DPPH), a free stable radical, was evaluated to elucidate the rate of possible lipid-derived radical scavenging in the mechanism of the enzyme inhibition. The results indicate that although the direct radical scavenging mechanism cannot be ruled out in the lipoxygenase inhibition by Mahonia aquifolium and its constituents, other mechanisms based on specific interaction between enzyme and alkaloids could play the critical role in the lipoxygenase inhibition rather than non-specific reactivity with free radicals. © 2007 Rackova et al; licensee BioMed Central Ltd.

引用

共 32 条

[1]

Muller K., Ziereis K., The antipsoriatic Mahonia aquifolium and its active constituents. I. Pro-antioxidant properties and inhibition of 5-lipoxygenase, Planta Med, 60, pp. 421-424, (1994)

[2]

Vollekova A., Kosalova D., Sochorova R., Isoquinoline alkaloids from Mahonia aquifolium stem bark are active against Malassezia spp, Folia Microbiol, 46, pp. 107-111, (2001)

[3]

Galle K., Muller-Jakic B., Proebstle A., Analytical and pharmacological studies on Mahonia aquifolium, Int J Phytomed, 1, pp. 59-62, (1994)

[4]

Amin A.H., Subhaiah T.V., Abbasi K.M., Berberine sulfate: Antimicrobial activity, bioassay, and mode of action, Can J Microbiol, 15, pp. 1067-1076, (1969)

[5]

Gieler U., Von Der Weth A., Heger M., Mahonia aquifolium - a new type of topical treatment for psoriasis, J Dermatol Treatment, 6, pp. 31-34, (1995)

[6]

Iwasa K., Moriyasu M., Tachibana Y., Kim H.S., Wataya Y., Wiegrebe W., Bastow K.F., Cosentino L.M., Kozuka M., Lee K.H., Simple isoquinoline and benzylisoquinoline alkaloids as potential antimicrobial, antimalarial, cytotoxic, and anti-HIV agents, Bioorg Med Chem, 9, pp. 2871-84, (2001)

[7]

Iwasa K., Moriyasu M., Yamori T., Turuo T., Lee D.U., Wiegrebe W., In vitro cytotoxicity of the protoberberine-type alkaloids, J Nat Prod, 64, pp. 896-98, (2001)

[8]

Cernakova M., Kost'Alova D., Kettmann V., Plodova M., Toth J., Drimal J., Potential antimutagenic activity of berberine, a constituent of Mahonia aquifolium, BMC Complementary and Alternative Medicine, 2, (2002)

[9]

Choi D.S., Kim S.J., Jung M.Y., Inhibitory activity of berberine on DNA strand cleveage induced by hydrogen peroxide and cytochrome c, Biosci Biotech Biochem, 65, pp. 452-455, (2001)

[10]

Yokozawa T., Ishida A., Kashiwada Y., Cho E.J., Kim H.Y., Ikeshiro Y., Coptidis rhizoma: Protective effects against peroxynitrite-induced oxidative damage and elucidation of its active components, J Pharm Pharmacol, 56, pp. 547-556, (2003)

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