Regeneration of Transgenic Plants from Electroporated Protoplasts of Asparagus officinalis L

被引：0
|
作者：
Sandip Mukhopadhyay
Serge Overney
Serge Yelle
Yves Desjardins
机构：
[1] University Laval,Centre de Recherche en Horticulture, Departement de Phytologie, F.S.A.A., Pavilion Envirotron
[2] University of Calcutta,Centre of Advanced Study, Department of Botany
[3] Univers1te de Genève,Laboratoire de Biochimie et de Physiologie Végétales
[4] 3place de I’Université,undefined
来源：
Journal of Plant Biochemistry and Biotechnology | 2002年 / 11卷
关键词：
Asparagus officinalis L; electroporation; protoplast; somatic embryogenesis; transgenic plants;
D O I：
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中图分类号：
学科分类号：
摘要：
An effective method for consistent regeneration of transgenic asparagus (Asparagus officinalis L) plants from electroporated protoplasts is described. Transgenic plants containing β-glucuronidase (GUS) and neomycin-phosphotransferase (NPT II) genes were obtained by electroporating callus-derived protoplasts of Asparagus officinalis L. Embryogenic callus tissue and plants from four kanamycin resistant lines expressed P-glucuronidase activity, as revealed by histological staining. The amplification of genomic DNA by polymerase chain reaction revealed the presence of both GUS and NPT II genes in transformed callus tissue and plants. Southern hybridization confirmed the integration of these genes into the asparagus genome.
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页码：57 / 60
页数：3
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