HPTLC Analysis of Venlafaxine Hydrochloride in the Bulk Drug and Tablets

A simple, precise, accurate, and rapid HPTLC method for quantitative analysis of venlafaxine hydrochloride in the tablet dosage form has been established and validated. Chromatographic separation was performed on 10 cm × 20 cm aluminum backed HPTLC plates coated with 0.2-mm layers of silica gel 60 F254, previously washed with methanol, with toluene-methanol 7:3.5 (v/v) as mobile phase. Densitometric evaluation was performed at 228 nm. Experimental conditions, for example size of band applied, chamber saturation time, solvent front migration, slit width, etc., were critically studied and the optimum conditions determined. The drug was satisfactorily resolved at RF 0.19. The validated calibration range was 400–2000 ng per band (r = 0.999). The suitability of the method for quantitative analysis of the compound was proved by validation in accordance with the requirements of pharmaceutical regulatory standards. The selectivity, accuracy, precision, and speed of the method make it useful for routine analysis of the pharmaceutical preparation in industrial quality-control and regulatory laboratories.