Identification a coat protein region of cucumber mosaic virus (CMV) essential for long-distance movement in cucumber

被引：0
作者：
Katalin Salánki
László Kiss
Ákos Gellért
Ervin Balázs
机构：
[1] Agricultural Biotechnology Center,Department of Plant Pathology
[2] Corvinus University of Budapest,undefined
[3] Agricultural Research Institute of the Hungarian Academy of Sciences,undefined
来源：
Archives of Virology | 2011年 / 156卷
关键词：
Coat Protein; Cucumber Mosaic Virus; Movement Protein; Phloem Exudate; Cucumber Mosaic Virus Strain;
D O I：
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中图分类号：
学科分类号：
摘要：
To characterise the long-distance movement determinant of cucumoviral coat proteins (CPs), five mutants were engineered into the CMV CP bearing the corresponding tomato aspermy virus (TAV) loops exposed on the surface of the virion. Both viruses can move long-distance in Nicotiana clevelandii, but only CMV can move long-distance in cucumber. Investigation of the CMV chimeras identified three amino acids of the βB-βC loop that were essential for the CMV long-distance movement in cucumber. Introducing these mutations into the TAV CP was not sufficient for long-distance movement, indicating that this is not the sole region causing long-distance movement deficiency.
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页码：2279 / 2283
页数：4
相关论文
共 82 条
[1]  
Ding B(1995)Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants Virology 207 345-353
[2]  
Li Q(2010)The solution structures of the Cucumber mosaic virus and Tomato aspermy virus coat proteins explored with molecular dynamics simulations J Mol Graph Model 28 569-576
[3]  
Nguyen L(1974)Comparative studies on tomato aspermy and cucumber mosaic viruses. I. Physical and chemical properties Virology 57 392-401
[4]  
Palukaitis P(2005)Evidence for interaction between the 2a polymerase protein and the 3a movement protein of Cucumber mosaic virus J Gen Virol 86 3171-3177
[5]  
Lucas WJ(1998)Characterization of cucumber mosaic virus. V. Cell-to-cell movement requires capsid protein but not virions Virology 246 221-231
[6]  
Gellért Á(1997)Characterization of cucumber mosaic virus. III. Localization of sequences in the movement protein controlling systemic infection in cucurbits Virology 230 343-349
[7]  
Balázs E(1996)Comparison of the nucleic acid- and NTP-binding properties of the movement protein of cucumber mosaic cucumovirus and tobacco mosaic tobamovirus Virology 216 71-79
[8]  
Habili N(2006)Analysis of the viability of coat-protein hybrids between Cucumber mosaic virus and Tomato aspermy virus J Gen Virol 87 2085-2088
[9]  
Francki RI(1972)Contribution á l’étude du virus de la mosaique du concombre (CMV). I. Méthode de purification rapide du virus Ann Phytopath 4 25-38
[10]  
Hwang MS(2002)The structure of tomato aspermy virus by X-ray crystallography J Struct Biol 139 90-102
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