Cell-free DNA in the plasma of patients with systemic sclerosis

被引：0
作者：
Marta Mosca
Tiziana Giuliano
Giovanna Cuomo
Marica Doveri
Chiara Tani
Michele Curcio
Giuseppina Abignano
Francesca De Feo
Laura Bazzichi
Alessandra Della Rossa
Gabriele Valentini
Stefano Bombardieri
机构：
[1] University of Pisa,Rheumatology Unit, Department of Internal Medicine
[2] Second University of Napoli,Rheumatology Unit
[3] U.O. Immunoematologia 2-Azienda Ospedaliero-Universitaria Pisana,undefined
来源：
Clinical Rheumatology | 2009年 / 28卷
关键词：
Biomarkers; Cell-free DNA; Disease activity; Plasma cell-free DNA; Scleroderma; Systemic autoimmune diseases;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of the present study was to evaluate the concentration of cell-free DNA (cf-DNA) in the plasma of patients with systemic sclerosis (SSc) and to examine the correlation of cf-DNA with clinical variables of the disease. The study population consisted of 122 SSc patients and 16 healthy controls. Epidemiological and clinical data were collected by direct assessment. The β-globin gene was used to determine the total amount of DNA in the plasma by real-time quantitative PCR analysis. cf-DNA was found in all patients (mean concentration 1,420.7 copies/ml) and controls (mean concentration 1,462.5), with no significant difference. In SSc patients, no correlation was found between cf-DNA and the type of organ involvement, but patients with active disease presented significantly higher cf-DNA concentrations than those with inactive disease (p < 0.05). Our data suggest that cf-DNA could provide a useful biomarker for the assessment of disease activity in SSc patients.
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页码：1437 / 1440
页数：3
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  • [1] Tong YW(2006)Diagnostic developments involving cell-free (circulating) nucleic acids Clin Chim Acta 363 187-196
  • [2] Lo YMD(2007)Circulating (cell-free) nucleic acids—a promising, non-invasive tool for early detection of several human diseases FEBS Lett 581 795-799
  • [3] Swarup V(2007)Circulating free DNA in plasma or serum as a biomarker of carcinogenesis: practical aspects and biological significance Mutat Res 635 105-117
  • [4] Rajeswari SW(2007)Levels of circulating cell-free serum DNA in benign and malignant breast lesions Int J Biol Markers 22 95-99
  • [5] Gormally E(2007)Elevated level of cell-free plasma DNA is associated with breast cancer Arch Gynecol Obstet 276 327-331
  • [6] Caboux E(1980)Free plasma DNA in patients with pulmonary embolism South Med J 73 345-346
  • [7] Vineis P(2000)Fluctuation of maternal and fetal free extracellular circulatory DNA in maternal plasma Obstet Gynecol 96 991-996
  • [8] Hainaut P(2001)Elevation of both maternal and fetal extracellular circulating deoxyribonucleic acid concentrations in the plasma of pregnant women with preeclampsia Am J Obstet Gynecol 184 414-419
  • [9] Zanetti-Dällenbach RA(2003)Time course of early and late changes in plasma DNA in trauma patients Clin Chem 49 1286-1291
  • [10] Schmid S(2007)Increased concentrations of antibody-bound circulatory cell-free DNA in rheumatoid arthritis Clin Chem 53 1609-1614
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