Cell-free DNA in the plasma of patients with systemic sclerosis

被引：0
作者：
Marta Mosca
Tiziana Giuliano
Giovanna Cuomo
Marica Doveri
Chiara Tani
Michele Curcio
Giuseppina Abignano
Francesca De Feo
Laura Bazzichi
Alessandra Della Rossa
Gabriele Valentini
Stefano Bombardieri
机构：
[1] University of Pisa,Rheumatology Unit, Department of Internal Medicine
[2] Second University of Napoli,Rheumatology Unit
[3] U.O. Immunoematologia 2-Azienda Ospedaliero-Universitaria Pisana,undefined
来源：
Clinical Rheumatology | 2009年 / 28卷
关键词：
Biomarkers; Cell-free DNA; Disease activity; Plasma cell-free DNA; Scleroderma; Systemic autoimmune diseases;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of the present study was to evaluate the concentration of cell-free DNA (cf-DNA) in the plasma of patients with systemic sclerosis (SSc) and to examine the correlation of cf-DNA with clinical variables of the disease. The study population consisted of 122 SSc patients and 16 healthy controls. Epidemiological and clinical data were collected by direct assessment. The β-globin gene was used to determine the total amount of DNA in the plasma by real-time quantitative PCR analysis. cf-DNA was found in all patients (mean concentration 1,420.7 copies/ml) and controls (mean concentration 1,462.5), with no significant difference. In SSc patients, no correlation was found between cf-DNA and the type of organ involvement, but patients with active disease presented significantly higher cf-DNA concentrations than those with inactive disease (p < 0.05). Our data suggest that cf-DNA could provide a useful biomarker for the assessment of disease activity in SSc patients.
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页码：1437 / 1440
页数：3
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