Learning to predict expression efficacy of vectors in recombinant protein production

[1] Baneyx F(1999)Recombinant protein expression in Escherichia coli Curr Opin Biotechnol 10 411-21

[2] Jonasson P(2002)Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli Biotechnol Appl Biochem 35 91-105

[3] Liljeqvist S(2005)Advanced genetic strategies for recombinant protein expression in Escherichia coli J Biotechnol 115 113-28

[4] Nygren PA(2002)High-throughput screening of soluble recombinant proteins Protein Sci 11 1714-9

[5] Stahl S(2005)Self-cleavage of fusion protein in vivo using TEV protease to yield native protein Protein Sci 14 936-41

[6] Sorensen HP(1996)Strategies for achieving high-level expression of genes in Escherichia coli Microbiol Rev 60 512-38

[7] Mortensen KK(2000)Structural proteomics: prospects for high throughput sample preparation Prog Biophys Mol Biol 73 339-45

[8] Shih YP(1988)Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 31-40

[9] Kung WM(1993)A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Biotechnology (N Y) 11 187-193

[10] Chen JC(1997)High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system Protein Expr Purif 10 309-19

[1] Baneyx F(1999)Recombinant protein expression in Escherichia coli Curr Opin Biotechnol 10 411-21

[2] Jonasson P(2002)Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli Biotechnol Appl Biochem 35 91-105

[3] Liljeqvist S(2005)Advanced genetic strategies for recombinant protein expression in Escherichia coli J Biotechnol 115 113-28

[4] Nygren PA(2002)High-throughput screening of soluble recombinant proteins Protein Sci 11 1714-9

[5] Stahl S(2005)Self-cleavage of fusion protein in vivo using TEV protease to yield native protein Protein Sci 14 936-41

[6] Sorensen HP(1996)Strategies for achieving high-level expression of genes in Escherichia coli Microbiol Rev 60 512-38

[7] Mortensen KK(2000)Structural proteomics: prospects for high throughput sample preparation Prog Biophys Mol Biol 73 339-45

[8] Shih YP(1988)Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 31-40

[9] Kung WM(1993)A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Biotechnology (N Y) 11 187-193

[10] Chen JC(1997)High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system Protein Expr Purif 10 309-19