Transgenic citrus plants expressing the p35 anti-apoptotic gene have altered response to abiotic stress

被引:0
作者
Vladimir Orbović
Jeb S. Fields
James P. Syvertsen
机构
[1] University of Florida/IFAS,Citrus Research and Education Center
[2] Virginia Polytechnic Institute and State University,Horticulture Department
来源
Horticulture, Environment, and Biotechnology | 2017年 / 58卷
关键词
Carrizo citrange; drought stress; environmental stress; freezing tolerance; leaf senescence; salinity stress;
D O I
暂无
中图分类号
学科分类号
摘要
Transgenic plants of the citrus rootstock Carrizo citrange cultivar [Citrus sinensis (L.) Osbeck × Poncirus trifoliata (L.) Raf.] expressing a p35 anti-apoptotic gene were exposed to three types of abiotic stress. The responses of plants from the three transgenic lines, p35-1, p35-2, and p35-4 were compared to those of wild-type (WT) plants, revealing consistent improvements in the ability of leaf explants from p35-2 transgenic plants to withstand freezing. When exposed to drought stress, plants transformed with p35 gene exhibited similar elongation growth and branching to WT plants. The stem water potential (SWP) in drought-stressed p35-2 and p35-4 plants was significantly lower than SWP measured in WT plants in one experiment, but in a repeat experiment, the SWP of WT and transgenic plants were similar. The ability to withstand salt stress was similar in all four genotypes. The introduction of anti apoptotic genes with beneficial effects in plant stress resilience into citrus, such as p35, may lead to the production of cultivars more tolerant to freezing conditions or other types of abiotic stress.
引用
收藏
页码:303 / 309
页数:6
相关论文
共 71 条
[1]  
Awada T(2003)Animal anti-apoptotic genes ameliorate the loss of turgor in water-stressed transgenic tobacco Can J Plant Sci 83 499-506
[2]  
Dunigan DD(2005)Managing salinity in citrus HortTechnology 15 108-113
[3]  
Dickman MB(1991)Cold-acclimation induced changes in freezing tolerance and translatable RNA content in Physiol Plant 82 519-522
[4]  
Boman BJ(2007) and Physiol Plant 130 532-542
[5]  
Zekri M(2009)Physiological responses of two citrus rootstock seedlings to flooding and drought stress J Horticult Sci Biotechnol 84 625-631
[6]  
Stover EW(1988)Orange varieties as interstocks increase salt tolerance of lemon trees Cryobiology 25 264-271
[7]  
Durham RE(2014)Changes in freezing tolerance and polypeptide content of spinach and citrus at 5°C HortScience 49 1010-1016
[8]  
Moore GA(2002)Freeze responses of Citrus and citrus-related genotypes in Florida field planting Proc Natl Acad Sci USA 99 15217-15221
[9]  
Guy CL(1992)Expression of the antiapoptotic baculovirus French). J Am Soc Hortic Sci 117 607-611
[10]  
García-Sánchez F(1999) gene in tomato blocks programmed cell death and provides broad spectrum resistance to disease Curr Biol 9 775-778