Localization and activity of calmodulin is involved in cell–cell adhesion of tumor cells and endothelial cells in response to hypoxic stress

被引:0
作者
W.-G. Shen
W.-X. Peng
Y. Shao
J.-F. Xu
G. Dai
Y. Zhang
F.-Y. Pan
C.-J. Li
机构
[1] Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology
[2] Yangzhou University,Medical College
[3] Nanjing Normal University,Jiangsu Key Laboratory for Molecular & Medical Biotechnology, Life Sciences College
来源
Cell Biology and Toxicology | 2007年 / 23卷
关键词
actin; Ca; /calmodulin; cell adhesion; hypoxia;
D O I
暂无
中图分类号
学科分类号
摘要
Adhesion of tumor cells to endothelial cells is known to be involved in the hematogenous metastasis of cancer, which is regulated by hypoxia. Hypoxia is able to induce a significant increase in free intracellular Ca2+ levels in both tumor cells and endothelial cells. Here, we investigate the regulatory effects of calmodulin (CaM), an intracellular calcium mediator, on tumor cell–endothelial cell adhesion under hypoxic conditions. Hypoxia facilitates HeLa cell–ECV304 endothelial cell adhesion, and results in actin cytoskeleton rearrangement in both endothelial cells and tumor cells. Suppression of CaM activation by CaM inhibitor W-7 disrupts actin cytoskeleton organization and CaM distribution in the cell–cell contact region, and thus inhibits cell–cell adhesion. CaM inhibitor also downregulates hypoxia-induced HIF-1-dependent gene expression. These results suggest that the Ca2+-CaM signaling pathway might be involved in tumor cell-endothelial cell adhesion, and that co-localization of CaM and actin at cell–cell contact regions might be essential for this process under hypoxic stress.
引用
收藏
页码:323 / 335
页数:12
相关论文
共 50 条
  • [1] Localization and activity of calmodulin is involved in cell-cell adhesion of tumor cells and endothelial cells in response to hypoxic stress
    Shen, W.-G.
    Peng, W. X.
    Shao, Y.
    Xu, J.-F.
    Dai, G.
    Zhang, Y.
    Pan, F.-Y.
    Li, C.-J.
    CELL BIOLOGY AND TOXICOLOGY, 2007, 23 (05) : 323 - 335
  • [2] Calmodulin is essential for angiogenesis in response to hypoxic stress in endothelial cells
    Shen, Wei-Gan
    Peng, Wan-Xin
    Dai, Gu
    Xu, Jian-Feng
    Zhang, Yu
    Li, Chao-Jun
    CELL BIOLOGY INTERNATIONAL, 2007, 31 (02) : 126 - 134
  • [3] EXPRESSION OF GALECTINS ON MICROVESSEL ENDOTHELIAL-CELLS AND THEIR INVOLVEMENT IN TUMOR-CELL ADHESION
    LOTAN, R
    BELLONI, PN
    TRESSLER, RJ
    LOTAN, D
    XU, XC
    NICOLSON, GL
    GLYCOCONJUGATE JOURNAL, 1994, 11 (05) : 462 - 468
  • [4] Differential Effects of Hypoxic Stress in Alveolar Epithelial Cells and Microvascular Endothelial Cells
    Signorelli, Sara
    Jennings, Paul
    Leonard, Martin O.
    Pfaller, Walter
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2010, 25 (01) : 135 - 144
  • [5] The cell adhesion molecule IGPR-1 is activated by and regulates responses of endothelial cells to shear stress
    Ho, Rachel Xi-Yeen
    Tahboub, Rawan
    Amraei, Razie
    Meyer, Rosana D.
    Varongchayakul, Nitinun
    Grinstaff, Mark
    Rahimi, Nader
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2019, 294 (37) : 13671 - 13680
  • [6] Adhesion of different cell cycle human hepatoma cells to endothelial cells and roles of integrin β1
    Song, Guan-Bin
    Qin, Jian
    Luo, Qing
    Shen, Xiao-Dong
    Yan, Run-Bin
    Cai, Shao-Xi
    WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (02) : 212 - 215
  • [8] Hypoxic suppression of the cell cycle gene CDC25A in tumor cells
    Hammer, Stefanie
    To, Kenneth K. -W.
    Yoo, Young-Gun
    Koshiji, Minori
    Huang, L. Eric
    CELL CYCLE, 2007, 6 (15) : 1919 - 1926
  • [9] A fibronectin-binding protein from rice bran with cell adhesion activity for animal tumor cells
    Shoji, Y
    Mita, T
    Isemura, M
    Mega, T
    Hase, S
    Isemura, S
    Aoyagi, Y
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (05) : 1181 - 1186
  • [10] Melanoma-associated antigen family protein-D1 regulation of tumor cell migration, adhesion to endothelium, and actin structures reorganization in response to hypoxic stress
    Shen, Wei-Gan
    Xue, Qing-Yu
    Wu, Yi-Ding
    Hu, Ben-Shun
    Zhu, Jun
    Zhang, Yu
    Su, Qing
    CELL COMMUNICATION AND ADHESION, 2007, 14 (01) : 21 - 31