Nicotine-induced human breast cancer cell proliferation attenuated by garcinol through down-regulation of the nicotinic receptor and cyclin D3 proteins

被引：0
作者：
Ching-Shyang Chen
Chia-Hwa Lee
Chang-Da Hsieh
Chi-Tang Ho
Min-Hsiung Pan
Ching-Shui Huang
Shih-Hsin Tu
Ying-Jan Wang
Li-Ching Chen
Yu-Jia Chang
Po-Li Wei
Yi-Yuan Yang
Chih-Hsiung Wu
Yuan-Soon Ho
机构：
[1] Taipei Medical University,Department of Surgery and Center of Quality Management and Breast Health Center
[2] Taipei Medical University,Graduate Institute of Medical Sciences
[3] Rutgers University,Department of Food Science
[4] National Kaohsiung Marine University,Department of Seafood Science
[5] Cathay General Hospital,Department of Surgery
[6] National Cheng Kung University Medical College,Department of Environmental and Occupational Health
[7] Taipei Medical University,Graduate Institute of Biomedical Technology
[8] Taipei Medical University-Shuang Ho Hospital,Department of Surgery, School of Medicine
[9] Taipei Medical University,Center of Excellence for Cancer Research
[10] Taipei Medical University,Graduate Institute of Clinical Medicine
来源：
Breast Cancer Research and Treatment | 2011年 / 125卷
关键词：
Smoking; Cyclin D3; Garcinol; Nicotinic acetylcholine receptors; Breast cancer;
D O I：
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摘要：
Previous studies have demonstrated that the persistent exposure of human bronchial epithelial cells to nicotine (Nic) through nicotinic acetylcholine receptors increases cyclin D1 promoter activity and protein expression. The main purpose of this study is to elucidate the carcinogenic role of cyclin D3, which is involved in breast tumorigenesis when induced by Nic. Real-time PCR analysis revealed that cyclin D3 is highly expressed at the mRNA level in surgically dissected breast tumor tissue, compared to the surrounding normal tissue (tumor/normal fold ratio = 17.93, n = 74). To test whether Nic/nicotinic acetylcholine receptor (nAChR) binding could affect cyclin D3 expression in human breast cancer cells, the transformed cell line MCF-10A-Nic (DOX) was generated from normal breast epithelial cells (MCF-10A) with inducible α9-nAChR gene expression, using the adenovirus tetracycline-regulated Tet-off system. Tet-regulated overexpression of α9-nAChR in MCF-10A-Nic (DOX) xenografted BALB/c-nu/nu mice resulted in a significant induction of cyclin D3. In contrast, cyclin D3 expression was down-regulated in α9-nAChR knock-down (siRNA) MDA-MB-231-xenografted tumors in NOD.CB17-PRKDC(SCID)/J(NOD-SCID) mice. Furthermore, we found that Nic-induced human breast cancer (MDA-MB-231) cell proliferation was inhibited by 1 μM of garcinol (Gar), isolated from the edible fruit Garcinia indica, through down-regulation of α9-nAChR and cyclin D3 expression. These results suggest that α9-nAChR-mediated cyclin D3 overexpression is important for nicotine-induced transformation of normal human breast epithelial cells. The homeostatic regulation of cyclin D3 has the potential to be a molecular target for antitumor chemotherapeutic or chemopreventive purposes in clinical breast cancer patients.
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页码：73 / 87
页数：14
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