Integrated lipid metabolomics and proteomics analysis reveal the pathogenesis of polycystic ovary syndrome

被引：22

作者：

Qian, Yu ^{[1
]}

Tong, Yun ^{[1
]}

Zeng, Yaqiong ^{[1
]}

Huang, Jingyu ^{[1
]}

Liu, Kailu ^{[1
]}

Xie, Ying ^{[1
]}

Chen, Juan ^{[1
]}

Gao, Mengya ^{[1
]}

Liu, Li ^{[1
]}

Zhao, Juan ^{[1
]}

Hong, Yanli ^{[1
]}

Nie, Xiaowei ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Prov Hosp Chinese Med, Dept Reprod Med, Affiliated Hosp, Nanjing 210029, Peoples R China

来源：

JOURNAL OF TRANSLATIONAL MEDICINE | 2024年 / 22卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Polycystic ovary syndrome; Follicular fluid; Granulosa cells; Lipid metabolomics; Proteomics; Biomarker; FOLLICULAR-FLUID; WOMEN; PCOS; CELLS;

D O I：

10.1186/s12967-024-05167-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background Polycystic ovary syndrome (PCOS) is an endocrinological and metabolic disorder that can lead to female infertility. Lipid metabolomics and proteomics are the new disciplines in systems biology aimed to discover metabolic pathway changes in diseases and diagnosis of biomarkers. This study aims to reveal the features of PCOS to explore its pathogenesis at the protein and metabolic level.Methods We collected follicular fluid samples and granulosa cells of women with PCOS and normal women who underwent in vitro fertilization(IVF) and embryo transfer were recruited. The samples were for the lipidomic study and the proteomic study based on the latest metabolomics and proteomics research platform.Results Lipid metabolomic analysis revealed abnormal metabolism of glycerides, glycerophospholipids, and sphingomyelin in the FF of PCOS. Differential lipids were strongly linked with the rate of high-quality embryos. In total, 144 differentially expressed proteins were screened in ovarian granulosa cells in women with PCOS compared to controls. Go functional enrichment analysis showed that differential proteins were associated with blood coagulation and lead to follicular development disorders.Conclusion The results showed that the differential lipid metabolites and proteins in PCOS were closely related to follicle quality,which can be potential biomarkers for oocyte maturation and ART outcomes.

引用

页数：19

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