Basophil activation test based on the expression of CD203c in the diagnostics of cow milk allergy in children

被引：0
作者：
Ciepiela O. [1 ]
Zwiazek J. [1 ]
Zawadzka-Krajewska A. [2 ]
Kotula I. [1 ]
Kulus M. [2 ]
Demkow U. [1 ]
机构：
[1] Department of Laboratory Diagnostics, Clinical Immunology of Developmental Age, Warsaw Medical University, 24 Marszalkowska St., Warsaw
[2] Department of Pediatric Pneumology and Allergology, Warsaw Medical University, Warsaw
来源：
European Journal of Medical Research | / 15卷 / Suppl 2期
关键词：
basophil activation test; CD203c; children; cow milk allergy; diagnosis;
D O I：
10.1186/2047-783X-15-S2-21
中图分类号：
学科分类号：
摘要：
Background: Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis. Objective: The aim of this study was to assess the potential of this technique for the diagnosis of pediatric cow milk allergy. Material and methods: The quantification of total and specific IgE and basophil activation test were performed to evaluate cow milk allergic (n = 9), and non-allergic children (n = 15). Results: Allergen-induced basophil activation was detected as a CD203c up-regulation. The expression of CD203c antigen on basophils was measured with flow cytometry. The antigen CD203c was detected on 15.4 ± 10.2% basophils from allergic children after incubation with specific allergens in concentration 1:10, whereas in the control group there were 3.0 ± 1.5% of basophils positive for this molecule (P < 0.05). Stimulation with allergen diluted 1:500 resulted in activation of 15.3 ± 11.2% of basophils in allergic children and 3.8 ± 2.3% of cells in the control group (P < 0.05). Positive results of an allergenicity test (above the cut- off level of 10%) were obtained in 7 out of the 9 allergic children. In 5 cases, the cutoff level was reached with both dilution of allergens (1:10 and 1:500). In 1 patient, positive stimulation was observed after stimulation with allergen diluted 1:10 and in another case only 1:500 resulted in stimulation of more then 10% of basophils. In no child of the control group, stimulation above 10% was noted. Conclusions: This study demonstrates that the analysis of allergen-induced CD203c up-regulation with flow cytometry is a reliable tool for the diagnosis of cow milk allergy in pediatric patients, with sensitivity similar to routine diagnostic tests and a higher specificity. © I. Holzapfel Publishers 2010.
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页码：21 / 26
页数：5
相关论文
共 25 条
[1]  
De Boissieu D., Dupont C., Time course of allergy to extensively hydrolyzed cow milk proteins in infants, J Pediatr, 136, pp. 119-120, (2000)
[2]  
De Boissieu D., Dupont C., Allergy to extensively hydrolyzed cow milk proteins in infants: Safety and duration of amino acid-based formula, J Pediatr, 41, pp. 271-273, (2002)
[3]  
Fiocchi A., Bouygue G.R., Restani P., Bonvini G., Startari R., Terracciano L., Accuracy of skin prick tests in IgE-mediated adverse reactions to bovine proteins, Ann Allergy Asthma Immunol, 89, 6, pp. 26-32, (2002)
[4]  
De Boissieu D., Waguet J.C., Dupont C., The atopy patch tests for detection of cow milk allergy with digestive symptoms, J Pediatr, 142, pp. 203-205, (2003)
[5]  
Sampson H.A., Ho D.G., Relationship between food-specific IgE concentrations and the risk of positive food challenges in children and adolescents, J Allergy Clin Immunol, 100, pp. 444-451, (1997)
[6]  
Isolauri E., Turjanmaa K., Combined skin prick and patch testing enhances identification of food allergy in infants with atopic dermatitis, J Allergy Clin Immunol, 97, pp. 9-15, (1996)
[7]  
Heine R.G., Elsayed S., Hosking C.S., Hill D.J., Cow milk allergy in infancy, Curr Opin Allergy Clin Immunol, 2, pp. 217-225, (2002)
[8]  
Hamilton R.G., Adkinson N.F., In vitro assays for the diagnosis of IgE-mediated disorders, J Allergy Clin Immunol, 114, pp. 213-225, (2004)
[9]  
Boumiza R., Debard A.L., Monneret G., The basophil activation test by flow cytometry: Recent developments in clinical studies, standardization and emerging perspectives, Clin Mol Allergy, 3, pp. 9-13, (2005)
[10]  
Buhring H.J., Seiffert M., Giesert C., Marxer A., Kanz L., Valent P., Sano K., The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3, Blood, 97, pp. 3303-3305, (2001)
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