A clinical case of enzootic bovine leukosis diagnosed by using clonal analysis of peripheral B lymphocytes in a Japanese Black cow

被引：3

作者：

Inokuma H. ^{[1
,2
]}

Nagata T. ^{[1
]}

Maezawa M. ^{[2
]}

Watanabe K.-I. ^{[3
]}

Kobayashi Y. ^{[3
]}

Kojima K. ^{[4
]}

Chambers J.K. ^{[4
]}

Uchida K. ^{[4
]}

机构：

[1] Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-Ku, Tokyo

[2] Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-Ku, Tokyo

[3] Laboratory of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro

[4] Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-Ku, Tokyo

来源：

Comparative Clinical Pathology | 2023年 / 32卷 / 2期

基金：

日本学术振兴会;

关键词：

B-cell clonality; Diagnosis; Enzootic bovine leucosis; Japanese Black;

D O I：

10.1007/s00580-022-03432-3

中图分类号：

学科分类号：

摘要：

A 7-year-old Japanese Black cow with anorexia was presented at a local veterinarian. As rectal palpation revealed an enlarged induration of the uterus, lymphoma was suspected, and the patient was introduced to the Veterinary Teaching Hospital of Obihiro University of Agriculture and Veterinary Medicine. Although lymphadenopathy and lymphocytosis were not found, a high proviral load of bovine leukemia virus (BLV) with 464 copies/10 ng DNA was recorded, and lymphocytes with constricted or flower-like nuclei were found occasionally in the peripheral blood; accordingly, enzootic bovine leukosis (EBL) was suspected. According to PCR for clonality analysis of B cells based on immunoglobulin heavy chain (IGH) gene rearrangement, monoclonal proliferation of peripheral blood B cells was evident, suggesting the presence of tumorigenic B cells in the peripheral blood and onset of EBL. Pathological examination including necropsy and histopathological examination confirmed the diagnosis of EBL. Clonality analysis for B cells in the peripheral blood is thought to be useful for diagnosing B-cell tumors such as EBL, especially in cases lacking lymphadenopathy and lymphocytosis. This is the first clinical case of EBL diagnosed by demonstrating monoclonal proliferation of peripheral blood B cells using IGH gene rearrangement-based PCR. © 2023, The Author(s).

引用

页码：195 / 200

页数：5

共 12 条

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