A molecular approach (multiplex polymerase chain reaction) for diagnosis of rhinosporidiosis

被引：2

作者：

Saha S. ^{[1
]}

Mondal D. ^{[2
]}

Khetawat D. ^{[2
]}

Roy A. ^{[3
]}

Chakrabarti S. ^{[2
]}

Bhattacharya B. ^{[4
]}

机构：

[1] Department of ENT, Medical College, Calcutta

[2] Dept. of Immunol. and Vaccine Devmt., Natl. Inst. of Cholera/Enteric Dis., Calcutta

[3] Dept. of Microbiol. and Parasitology, University College of Medicine, Calcutta

[4] Dept. of Biochem. and Biophysics, University College of Medicine, Calcutta

来源：

Indian Journal of Otolaryngology and Head and Neck Surgery | 2002年 / 54卷 / 4期

关键词：

Multiplex PCR; R; seeberi;

D O I：

10.1007/BF02993739

中图分类号：

学科分类号：

摘要：

A new multiplex PCR have been developed in our laboratory using primer sets, aiming amplification of both DNA target fragments obtained in 18S rRNA of commonly encountered fungi in human being and in Rhinosporidium seeberi using F1-fw/F2-rev (500 bp target) and Rhino-fw/ Rhino-rev (377 bp target). This multiplex PCR has been found to be able to detect R. seeberi from clinical samples and differentiate it from other fungi. Furthermore, by this multiplex PCR, R. seeberi, phylogenitically appears to belong to a member of so called DRIPs clade of fish parasite not a cyanobacterium as claimed previously, by some workers.

引用

页码：264 / 267

页数：3

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