Lack of correlation between DNA copy number and mRNA expression levels of c-myc in γ-radiation-induced mouse thymic lymphomas by using quantitative real-time PCR

被引：1

作者：

Santos J. ^{[1
]}

Vaquero C. ^{[1
]}

Reyes J. ^{[1
]}

López-Nieva P. ^{[1
]}

Matabuena M. ^{[1
]}

Villa M. ^{[1
]}

Fernández P. ^{[1
]}

Fernández-Piqueras J. ^{[1
]}

机构：

[1] Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid 28049, Crta. de Colmenar Viejo

来源：

Clinical and Translational Oncology | 2006年 / 8卷 / 5期

关键词：

c-myc; Gamma-irradiation; Genedosage; Mouse thymic lymphomas; mRNA levels; Real-time PCR;

D O I：

10.1007/s12094-006-0181-y

中图分类号：

学科分类号：

摘要：

Background: It is well documented that over-expression of the c-myc proto-oncogene occurs in the vast majority of mouse thymic lymphomas induced by γ-irradiation, evidencing the importance of this gene in T-cell lymphomagenesis. However, it remains unknown whether elevated levels of c-myc expression are driven by extra c-myc copy numbers. Materials and methods: Here we use a quantitative test on the basis of real-time PCR to determine the cellular copy number of c-myc in a set of 14 g-radiation-induced thymic lymphomas obtained from (C57BL/6J x BALB/ cJ) F1 hybrid mice with increased mRNA c-myc expression. Results: Since 5 out of 14 (35.7%) cases had no extra copy numbers of c-myc, gene amplification was obviously not the cause of c-myc over-expression in these tumours. In the remaining 9 tumours, c-myc over-expression was also accompanied with extra DNA copy numbers. Therefore, c-myc amplification might be a consequence of the genomic instability subsequent to the up-regulation of c-myc. However, linear regression analysis showed a lack of correlation between increasing DNA copy numbers and mRNA over expression of c-myc in these tumours (r = 0.029, p = 0.94). Conclusion: De-regulation of c-myc does not necessarity imply amplification of this gene in these tumours. This report is, to our knowledge, the first one comparing c-myc amplification with expression in lymphomas of the T-cell lineage. © FESEO 2006.

引用

页码：349 / 353

页数：4

共 26 条

[1]

Vennstrom B., Sheiness D., Zabielski J., Bishop J.M., Isolation and characterization of c-myc, a cellular homolog of the oncogene (v-myc) of avian myelocytomatosis virus strain 29, J Virol, 42, pp. 773-779, (1982)

[2]

Dalla-Favera R., Bregni M., Erikson J., Patterson D., Gallo R.C., Croce C.M., Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells, Proc Natl Acad Sci USA, 79, pp. 7824-7827, (1982)

[3]

Spencer C.A., Groudine M., Control of c-myc regulation in normal and neoplastic cells, Adv Cancer Res, 56, pp. 1-48, (1991)

[4]

McMorrow L.E., Newcomb E.W., Pellicer A., Identification of a specific marker chromosome early in tumour development in gamma-irradiated C57BL/6J mice, Leukemia, 2, pp. 115-119, (1988)

[5]

Muto M., Chen Y., Kubo E., Mita K., Analysis of early initiating event(s) in radiation-induced thymic lymphomagenesis, Jpn J Cancer Res, 87, pp. 247-257, (1996)

[6]

Vasmel W.L., Matthews E.A., Gillis C.P., Et al., Distinct chromosomal abnormalities in murine leukemia virus-induced T- and B-cell lymphomas, Int J Cancer, 43, pp. 1112-1119, (1989)

[7]

Gaudet F., Hodgson J.G., Eden A., Et al., Induction of tumours in mice by genomic hypomethylation, Science, 300, pp. 489-492, (2003)

[8]

Liyanage M., Weaver Z., Barlow C., Et al., Abnormal rearrangement within the alpha/delta T-cell receptor locus in lymphomas from Atm -deficient mice, Blood, 96, pp. 1940-1946, (2000)

[9]

Silva S., Babonits M., Wiener F., Klein G., Further studies on chromosome 15 trisomy in murine T-cell lymphomas: Mapping of the relevant chromosome segment, Int J Cancer, 41, pp. 738-743, (1988)

[10]

Banerjee M., Wiener F., Spira J., Et al., Mapping of the c-myc, pvt-1 and immunoglobulin kappa genes in relation to the mouse plasmacytoma-associated variant (6

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