Interaction between southern rice black-streaked dwarf virus minor core protein P8 and a rice zinc finger transcription factor

被引:0
作者
Jing Li
Nian-Jun Cai
Jin Xue
Jian Yang
Jian-Ping Chen
Heng-Mu Zhang
机构
[1] Zhejiang Academy of Agricultural Sciences,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, MOA and Zhejiang Province, Institute of Virology and Biotechnology
[2] Zhejiang Normal University,College of Chemistry and Life Science
[3] Hunan Agricultural University,College of Plant Protection
来源
Archives of Virology | 2017年 / 162卷
关键词
Rice Protoplast; Bimolecular Fluorescence Complementation; Living Plant Cell; Prey Protein; BiFC Assay;
D O I
暂无
中图分类号
学科分类号
摘要
The fijivirus southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in China and Vietnam. To better understand the molecular basis of SRBSDV infection, a yeast two-hybrid screen of a rice cDNA library was carried out using P8, a minor core protein of SRBSDV, as the bait. A rice Cys2His2-type zinc finger protein (OsZFP) was found to interact with SRBSDV P8. A strong interaction between SRBSDV P8 and OsZFP was then confirmed by pull-down assays, and bimolecular fluorescence complementation assays showed that the in vivo interaction was specifically localized in the nucleus of plant cells. Using a series of deletion mutants, it was shown that both the NTP-binding region of P8 and the first two zinc fingers of OsZFP were crucial for their interaction in plant cells. The localization in the nucleus and activation of transcription in yeast supports the notion that OsZFP is a transcription factor. SRBSDV P8 may play an important role in fijiviral infection and symptom development by interfering with the host transcription activity of OsZFP.
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页码:1261 / 1273
页数:12
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