Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

被引：0

作者：

S. Padilla

U. C. Tran

M. Jiménez-Hidalgo

J. M. López-Martín

A. Martín-Montalvo

C. F. Clarke

P. Navas

C. Santos-Ocaña

机构：

[1] Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER),Centro Andaluz de Biología del Desarrollo

[2] UCLA,Department of Chemistry and Biochemistry

来源：

Cellular and Molecular Life Sciences | 2009年 / 66卷

关键词：

Ubiquinone; coenzyme Q; mitochondria; yeast regulation;

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学科分类号：

摘要：

Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.

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