Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

被引：0
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作者：
S. Padilla
U. C. Tran
M. Jiménez-Hidalgo
J. M. López-Martín
A. Martín-Montalvo
C. F. Clarke
P. Navas
C. Santos-Ocaña
机构：
[1] Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER),Centro Andaluz de Biología del Desarrollo
[2] UCLA,Department of Chemistry and Biochemistry
来源：
Cellular and Molecular Life Sciences | 2009年 / 66卷
关键词：
Ubiquinone; coenzyme Q; mitochondria; yeast regulation;
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摘要：
Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
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