Survival of Burkholderia pseudomallei in Water

被引：25

作者：

Moore R.A. ^{[1
]}

Tuanyok A. ^{[2
]}

Woods D.E. ^{[1
]}

机构：

[1] Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary

[2] Microbial Genetics and Genomics Center, Northern Arizona University, Flagstaff

来源：

BMC Research Notes | / 1卷 / 1期

基金：

加拿大健康研究院;

关键词：

Luria Broth; Deficient Mutant; Melioidosis; Miliary Tuberculosis; Burkholderia Pseudomallei;

D O I：

10.1186/1756-0500-1-11

中图分类号：

学科分类号：

摘要：

Background. The ability of Burkholderia pseudomallei to survive in water likely contributes to its environmental persistence in endemic regions. To determine the physiological adaptations which allow B. pseudomallei to survive in aqueous environments, we performed microarray analyses of B. pseudomallei cultures transferred from Luria broth (LB) to distilled water. Findings. Increased expression of a gene encoding for a putative membrane protein (BPSL0721) was confirmed using a lux-based transcriptional reporter system, and maximal expression was noted at approximately 6 hrs after shifting cells from LB to water. A BPSL0721 deficient mutant of B. pseudomallei was able to survive in water for at least 90 days indicating that although involved, BPSL0721 was not essential for survival. BPSL2961, a gene encoding a putative phosphatidylglycerol phosphatase (PGP), was also induced when cells were shifted to water. This gene is likely involved in cell membrane biosynthesis. We were unable to construct a PGP mutant suggesting that the gene is not only involved in survival in water but is essential for cell viability. We also examined mutants of polyhydroxybutyrate synthase (phbC), lipopolysaccharide (LPS) oligosaccharide and capsule synthesis, and these mutations did not affect survival in water. LPS mutants lacking outer core were found to lose viability in water by 200 days indicating that an intact LPS core provides an outer membrane architecture which allows prolonged survival in water. Conclusion. The results from these studies suggest that B. pseudomallei survival in water is a complex process that requires an LPS molecule which contains an intact core region. © 2008 Moore et al; licensee BioMed Central Ltd.

引用

共 20 条

[1]

Chaowagul W., White N.J., Dance D.A., Wattanagoon Y., Naigowit P., Davis T.M., Looareesuwan S., Pitakwatchara N., Melioidosis: A major cause of community-acquired septicemia in northeastern Thailand, J Infect Dis, 159, pp. 890-899, (1989)

[2]

Dance D.A.B., Melioidosis: The tip of the iceberg?, Clinical Microbiology Reviews, 4, pp. 52-60, (1991)

[3]

Smith C., Allen J.C., Embi N.M., Othman O., Razak N., Ismail G., Human melioidosis: An emergingmedical problem, MIRCEN Journal of Applied Microbiology Technology, 3, pp. 3443-3366, (1987)

[4]

Sanford J.P., Pseudomonas species (including melioidosis and glanders), Principles and Practiceof Infectious Diseases, (1995)

[5]

Smith M.D.V., Wuthiekanun A.L., White. N.J., Quantitative discovery of Burkholderia pseudomallei from soil in Thailand, Trans R Soc Trop Med Hyg, 89, pp. 488-490, (1995)

[6]

Whitmore A., Krishnaswami C.S., An account of the discovery of a hitherto undescribed infective disease occurring amoung the population of Rangoon, Indian Medical Gazette, 47, pp. 262-267, (1912)

[7]

Wuthiekanun V., Smith M.D., Dance D.A., White N.J., Isolation of Pseudomonas pseudomallei from soil in north-eastern Thailand, Trans R Soc Trop Med Hyg, 89, pp. 41-43, (1995)

[8]

Wuthiekanun V., Smith M.D., White N.J., Survival of Burkholderia pseudomallei in the absence of nutrients, Trans R Soc Trop Med Hyg, 89, (1995)

[9]

Currie B.J., Mayo M., Anstey N.M., Donohoe P., Haase A., Kemp D.J., A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates, Am J Trop Med Hyg, 65, pp. 177-179, (2001)

[10]

Inglis T.J., Garrow S.C., Adams C., Henderson M., Mayo M., Currie B.J., Acute melioidosis outbreak in Western Australia, Epidemiol Infect, 123, pp. 437-443, (1999)

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