First Observation of Surface Plasmon-Coupled Emission Due to LED Excitation

被引:0
作者
Derek S. Smith
Yordan Kostov
Govind Rao
Ignacy Gryczynski
Joanna Malicka
Zygmunt Gryczynski
Joseph R. Lakowicz
机构
[1] University of Maryland at Baltimore County,Department of Chemical and Biochemical Engineering, Center for Advanced Sensor Technology
[2] University of Maryland at Baltimore,Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy
来源
Journal of Fluorescence | 2005年 / 15卷
关键词
Surface plasmons; directional emission; LED excitation; fluorescence; thin metal films;
D O I
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中图分类号
学科分类号
摘要
Detection limitations for fluorescence methods are normally determined by the phenomenon itself rather than the sensitivity of the instrumentation. These limitations make it necessary to have high sensitivity, high cost equipment causing fluorescence methods to remain lab-oriented. Alleviation of the limitations can be achieved through the phenomenon of surface plasmon-coupled emission (SPCE), which displays enhanced, directional, polarized fluorescence. Here we present the excitation of SPCE from Rhodamine B with a light-emitting diode (LED). Incorporating the gains in sensitivity due to SPCE with LED excitation, it could be possible to design low-cost, high-sensitivity sensors that would allow measurements to be performed in the field.
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页码:895 / 900
页数:5
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