RAPD Characterization of Single Females of the Root-knot Nematodes, Meloidogyne Spp.

被引：0
|
作者：
Onivaldo Randig
Frédéric Leroy
Philippe Castagnone-Sereno
机构：
[1] INRA,Unité Interactions Plantes
[2] INRA,Microorganismes et Santé Végétale
来源：
European Journal of Plant Pathology | 2001年 / 107卷
关键词：
fingerprint; PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Random amplified polymorphic DNA fingerprinting was performed on single females of root-knot nematodes, Meloidogyne spp., using a new procedure for DNA isolation. One-fourth of the total DNA amount isolated from a single female proved to be sufficient as a template in a polymerase chain reaction. Electrophoretic patterns of the amplified fragments were reproducible between replicates from a single female or sister females from the same progeny, and identical to those obtained with genomic DNA purified from a large number of nematodes. Moreover, a comparative analysis over three successive generations showed stability of the amplification patterns, thus demonstrating the utility of this procedure for epidemiological and ecological studies on root-knot nematodes.
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页码：639 / 643
页数：4
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