Immunolocalization of iron regulatory protein expression in the murine central nervous system

被引：0
作者：
Leibold E.A. [1 ,2 ,4 ]
Gahring L.C. [1 ,2 ]
Rogers S.W. [1 ,3 ,4 ]
机构：
[1] Eccles Program in Human Molecular Biology and Genetics, University of Utah, HMBG 15 N, Salt Lake City, UT 84112
[2] Department of Medicine, University of Utah, Salt Lake City
[3] Department of Neurobiology and Anatomy, University of Utah, Salt Lake City
[4] Geriatric Research, Education and Clinical Center, Veterans Affairs Medical Center, Salt Lake City
来源：
Histochemistry and Cell Biology | 2001年 / 115卷 / 3期
基金：
美国国家卫生研究院;
关键词：
Brain; Immunolocalization; Iron; Iron regulatory proteins (IRPs); Mouse;
D O I：
10.1007/s004180000246
中图分类号：
学科分类号：
摘要：
We examined the expression of the iron regulatory proteins 1 and 2 (IRP1 and IRP2) in the brains of adult (4-6 months) CBA/J mice. Anti-IRP1 immunoreactivity was localized to cell bodies, including putative neurons and oligodendrocytes. In contrast, anti-IRP2 staining was prevalent throughout the neuropil of regions of the brain consistent with the central autonomic network (CAN) and mossy fibers emanating from hippocampal dentate granule cells. Essentially no staining for IRP2 was observed in the cerebellum in contrast to strong IRP1 immunoreactivity in Purkinje cells. Notably, cells within one vestibular nucleus exhibited staining by both IRP1 and IRP2. Our results suggest distinct roles for IRP1 and IRP2 in the regulation of iron homeostasis in the mammalian nervous system where IRP1 may provide a maintenance function in contrast to IRP2 that could participate in modulating proper CAN functions, including cardiopulmonary, gustatory as well as fine motor control.
引用
收藏
页码：195 / 203
页数：8
相关论文
共 31 条
[1]  
Benarroch E.E., The central autonomic network: Functional organization, dysfunction, and perspective, Mayo Clin Proc, 68, pp. 988-1001, (1993)
[2]  
Benkovic S.A., Connor J.R., Ferritin, transferrin, and iron in selected regions of the adult and aged rat brain, J Comp Neurol, 338, pp. 97-113, (1993)
[3]  
Bush A.I., Metals and neuroscience, Curr Opin Chem Biol, 4, pp. 184-191, (2000)
[4]  
Butt J., Kim H.-Y., Basilion J.P., Cohen S., Iwai K., Philpott C.C., Altschul S., Klausner R.D., Rouault T.A., Differences in the RNA binding sites of iron regulatory proteins and potential target diversity, Proc Natl Acad Sci USA, 93, pp. 4345-4349, (1996)
[5]  
Choi D.W., Koh J.Y., Zinc and brain injury, Ann Rev Neurosci, 21, pp. 347-375, (1998)
[6]  
Cole T.B., Wenzel H.J., Kafer K.E., Schwartzkroin P.A., Palmiter R.D., Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene, Proc Natl Acad Sci USA, 96, pp. 1716-1721, (1999)
[7]  
Connor J.R., Menzies S.L., Relationship of iron to oligodendrocytes and myelination, Glia, 17, pp. 83-93, (1996)
[8]  
Felt B.T., Lozoff B., Brain iron and behavior of rats are not normalized by treatment of iron deficiency anemia during early development, J Nutr, 126, pp. 693-701, (1996)
[9]  
Frederickson C.J., Neurobiology of zinc and zinc-containing neurons, Int Rev Neurobiol, 131, pp. 145-238, (1989)
[10]  
Gahring L.C., Carlson N.G., Kulmar R.A., Rogers S.W., Neuronal expression of tumor necrosis factor alpha in the murine brain, Neuroimmunomodulation, 5, pp. 289-303, (1996)
1234