Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA

被引：0

作者：

Motoyasu Minami

Kaori Nishio

Yui Ajioka

Hiroaki Kyushima

Katsuki Shigeki

Kaneo Kinjo

Kazuno Yamada

Masashi Nagai

Kiyoshi Satoh

Yozo Sakurai

机构：

[1] Chubu University,Department of Environmental Biology, College of Bioscience and Biotechnology

[2] National Institute of Biomedical Innovation,Tanegashima Division, Research Center for Medicinal Plant Resources

[3] Okinawa Agricultural Experiment Station,Laboratory of Root and Tuber Crop, Horticultural Branch

[4] Kinjirushi Co.,R&D Division

[5] Ltd,JICA

[6] 1–211 Kitamatsuyama,undefined

来源：

Journal of Natural Medicines | 2009年 / 63卷

关键词：

Chloroplast DNA;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.

引用

页码：75 / 79

页数：4

共 42 条

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