Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording

被引：0
作者：
Julien Amendola
Norah Boumedine
Marion Sangiardi
Oussama El Far
机构：
[1] INSERM,
[2] Aix-Marseille Université,undefined
来源：
Scientific Reports | / 5卷
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摘要：
Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.
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[11]  
Bernheim L(1989)The cholinergic neuronal differentiation factor from heart cells is identical to leukemia inhibitory factor Science 246 1412-6
[12]  
Mathie A(1992)Effects of ciliary neurotrophic factor (CNTF) and depolarization on neuropeptide expression in cultured sympathetic neurons Dev Biol 150 281-93
[13]  
Hille B(1978)Adrenergic differentiation of cells of the cholinergic ciliary and Remak ganglia in avian embryo after Proc Natl Acad Sci USA 75 2030-4
[14]  
Vivas O(1980) transplantation J Exp Biol 89 57-71
[15]  
Castro H(2013)Dual function during development of rat sympathetic neurones in culture Journal of Biological Chemistry 288 4637-4648
[16]  
Arenas I(2000)Ca2+-independent Activation of Ca2+/Calmodulin-dependent Protein Kinase II Bound to the C-terminal Domain of CaV2.1 Calcium Channels Brain Res Bull 53 635-47
[17]  
Elias-Vinas D(2003)Perinatal development of lumbar motoneurons and their inputs in the rat J Physiol 549 21-36
[18]  
Garcia DE(2006)A-, T- and H-type currents shape intrinsic firing of developing rat abducens motoneurons J Neurosci 26 11720-5
[19]  
Wanke E(1986)GABAergic input onto CA3 hippocampal interneurons remains shunting throughout development Brain Res 378 197-202
[20]  
Ma H(1993)Development of the fast, transient outward K+ current in embryonic sympathetic neurones J Neurosci 13 2591-600
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