Rapid Analysis of Hemoglobins in Whole Blood by a Light Scattering Method

被引：0
作者：
S. A. Lisenko
M. M. Kugeiko
机构：
[1] Belorussian State University,
来源：
Journal of Applied Spectroscopy | 2013年 / 80卷
关键词：
whole blood; erythrocytes; morphological parameters; biochemical composition; transmittance spectrum;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed a method for fast determination of blood morphological and biochemical parameters (concentration of oxyhemoglobin, deoxyhemoglobin, methemoglobin, and sulfhemoglobin; the hematocrit; average concentration of total hemoglobin in erythrocytes; the erythrocyte parameter associated with their volume and shape) from the diffuse transmittance spectrum of light for a thin cuvet containing whole blood (thickness 200 μm), illuminated by collimated light. The method is based on an analytical dependence of the spectral diffuse transmission coefficient of blood on the parameters of interest, taking into account multiple scattering of light by erythrocytes and possible variations in their shape. We have analyzed the uncertainties of the method when the analyte parameters are all variable.
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页码：419 / 428
页数：9
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