Neuro-protective effects of Ligustri Fructus by suppression of oxidative stress in mouse model of Parkinson’s disease

被引：3

作者：

Ye M. ^{[1
]}

Kim M. ^{[1
]}

Bae H. ^{[1
]}

机构：

[1] Department of Physiology, College of Korean Medicine, Kyung Hee University, #1, Hoegi-dong, Dongdaemungu, 130-701, Seoul

来源：

Oriental Pharmacy and Experimental Medicine | 2016年 / 16卷 / 2期

基金：

新加坡国家研究基金会;

关键词：

Ligustri Fructus; MPTP; Parkinson’s disease; PC12; cell; ROS;

D O I：

10.1007/s13596-016-0223-0

中图分类号：

学科分类号：

摘要：

Parkinson’s disease (PD) is a progressive degenerative disorder of the central nervous system (CNS) that leads to impairment of motor skills and speech. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes damage to the dopaminergic (DA) neurons, and 1-4-Methyl-4-phenylpyridinium (MPP+) causes cell death in differentiated PC12 cells that is similar to the degeneration that occurs in PD. Moreover, MPTP treatment increases the activity of the microglia cells that produced reactive oxygen species (ROS). We recently reported that Ligustri Fructus (LF), a widely used traditional herbal medicine, increases cell viability in a yeast model of PD. In the present study, we examined the inhibitory effect of LF (0.01, 5, 10 ug) on the neurotoxicity of MPTP in mice and on the MPP + -induced cell death in differentiated PC12 cells. In vivo experiment, MPTP injection revealed a significant loss of DA neurons in the substantia nigra, while LF (100, 200 mg/ kg) treatment dramatically reversed DA neuron loss in immunohistochemistry assay for tyrosine hydroxylase (TH). Furthermore, LF attenuated the MPP + -induced cell death, decreased the generation of ROS, and activated glutathione peroxidase in PC12 cells. These results suggest that LF may be beneficial for the treatment of neurodegenerative diseases such as PD. © 2016, Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media Dordrecht.

引用

页码：123 / 129

页数：6

共 39 条

[31]

Vitvitsky V., Thomas M., Ghorpade A., Gendelman H.E., Banerjee R., A functional transsulfuration pathway in the brain links to glutathione homeostasis, J Biol Chem, 281, pp. 35785-35793, (2006)

[32]

Wang S.S., Chen J.H., Liu X.J., Preliminary study on pharmacologic action of Ligustrum japonicum, Zhongguo Zhong Xi Yi Jie He Za Zhi, 14, pp. 670-672, (1994)

[33]

Wang Z., Fang J.N., Ge D.L., Li X.Y., Chemical characterization and immunological activities of an acidic polysaccharide isolated from the seeds of Cuscuta chinensis, Lam Acta Pharmacol Sin, 21, pp. 1136-1140, (2000)

[34]

Wang X.M., Fu H., Liu G.X., Effect of wuzi yanzong pill and its disassembled prescription on mitochondrial DNA deletion, respiratory chain complexes and ATP synthesis in aged rats, Zhongguo Zhong Xi Yi Jie He Za Zhi, 21, pp. 437-440, (2001)

[35]

Wu D.C., Teismann P., Tieu K., Vila M., Jackson-Lewis V., Ischiropoulos H., Przedborski S., NADPH oxidase mediates oxidative stress in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson’s disease, Proc Natl Acad Sci U S A, 100, pp. 6145-6150, (2003)

[36]

Yang H.M., Shin H.K., Kang Y.H., Kim J.K., Cuscuta chinensis extract promotes osteoblast differentiation and mineralization in human osteoblast-like MG-63 cells, J Med Food, 12, pp. 85-92, (2009)

[37]

Zeevalk G.D., Bernard L.P., Albers D.S., Mirochnitchenko O., Nicklas W.J., Sonsalla P.K., Energy stress-induced dopamine loss in glutathione peroxidase-overexpressing transgenic mice and in glutathione-depleted mesencephalic cultures, J Neurochem, 68, pp. 426-429, (1997)

[38]

Zhang Z.T., Cao X.B., Xiong N., Wang H.C., Huang J.S., Sun S.G., Wang T., Morin exerts neuroprotective actions in Parkinson disease models in vitro and in vivo, Acta Pharmacol Sin, 31, pp. 900-906, (2010)

[39]

Zhang J.F., Et al., Aqueous extracts of Fructus Ligustri Lucidi enhance the sensitivity of human colorectal carcinoma DLD-1 cells to doxorubicin-induced apoptosis via Tbx3 suppression, Integr Cancer Ther, 10, pp. 85-91, (2011)

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