Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis

被引：0
|
作者：
E. Yu. Yagudaeva
L. S. Zhigis
O. A. Razgulyaeva
V. S. Zueva
E. E. Melnikov
V. P. Zubov
L. V. Kozlov
A. M. Bichucher
O. V. Kotel’nikova
A. P. Alliluev
A. E. Avakov
L. D. Rumsh
机构：
[1] Russian Academy of Sciences,Shemyakin
[2] FGUN Gabrichevskii Moscow Research Institute of Epidemiology and Microbiology,Ovchinnikov Institute of Bioorganic Chemistry
[3] Russian University of Peoples’ Friendship,Medical Faculty
[4] FGUN Branch (NPO Microgen),Gabrichevskii Plant for the Production of Bacterial Preparations
来源：
Russian Journal of Bioorganic Chemistry | 2010年 / 36卷
关键词：
IgA1 protease; meningococcus; isolation; enzymatic activity; immunogenicity;
D O I：
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学科分类号：
摘要：
A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and precipitate obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been developed. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 µg. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.
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页码：81 / 89
页数：8
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